SuperSignal MaxiSignal Maximum Sensitivity ECL Substrate - 36224ES
SuperSignal MaxiSignal Maximum Sensitivity Substrate is designed to detect antibodies and associated antigens directly or indirectly labeled with horseradish peroxidase (HRP). The principle is that proteins or nucleic acids were transferred to the imprinted membrane after electrophoresis, and the target proteins on the membrane were bound by primary antibody and secondary antibody labeled with HRP, or the nucleic acids on the membrane were bound directly or indirectly by probes labeled with HRP. After washing the membrane, the ECL working solution prepared by the product was used to incubate the membrane at room temperature for several minutes. The imprinted membrane was wrapped with plastic wrap and fixed to the X-ray exposure Cassette. Then the X-ray film is pressed on the membrane in a darkroom and exposed for several seconds to several hours. After development and fixing, protein or nucleic acid bands can be clearly displayed on the X-ray film.

This kit has a unique luminescent/enhanced substrate system, While reducing the exposure background, the introduction of a new oxidant greatly improves the stability of the kit, which can achieve low fick-grade western blotting detection. In addition to X-rays, can also be detected by the automatic imaging system.

Applications:
- Chemiluminescence ELISA - Western Blot - Dot Blot-DNA/RNA - Southern blot-DNA - Northern blot-RNA

Packaging: 10mL / 100mL / 200mL / 500mL

Selected citations:
[1] CircRNA circ_0006156 inhibits the metastasis of prostate cancer by blocking the ubiquitination of S100A9 [published online ahead of print, 2022 Jun 27]. Cancer Gene Ther. 2022;10.1038/s41417-022-00492-z. doi:10.1038/s41417-022-00492-z(IF:5.987)
[2] CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer. Cell Death Discov. 2022;8(1):152. Published 2022 Apr 2. doi:10.1038/s41420-022-00965-y(IF:5.241)
[3] Phenotypic and genetic characterisation of an emerging reovirus from Pekin ducks in China. Sci Rep. 2019;9(1):7784. Published 2019 May 23. doi:10.1038/s41598-019-44178-3(IF:4.011)

Features:

High sensitivity and high signal-to-noise ratio, it can detect low fick-grade antigens
Long duration of luminescence, fluorescence can make X-ray film sensitive for more than 8 hours, especially suitable for detecting trace protein or nucleic acid
Higher dilution ratio antibodies can be used to greatly save antibodies
Dilution ratio of primary antibody (liquid storage concentration 1mg/mL) : 1:1000-1:100000
Dilution ratio of secondary antibody (liquid storage concentration 1mg/mL): 1:100000-1:500000

SuperSignal SuperDura Extended Duration ECL Substrate - 36223ES
YEASEN ECL series luminescence Kit is designed to detect antibodies and associated antigens directly or indirectly labeled with horseradish peroxidase (HRP). The principle is that proteins or nucleic acids were transferred to the imprinted membrane after electrophoresis, and the target proteins on the membrane were bound by primary antibody and secondary antibody labeled with HRP, or the nucleic acids on the membrane were bound directly or indirectly by probes labeled with HRP. After washing the membrane, the ECL working solution prepared by the product was used to incubate the membrane at room temperature for several minutes. The imprinted membrane was wrapped with plastic wrap and fixed to the X-ray exposure Cassette. Then the X-ray film is pressed on the membrane in a darkroom and exposed for several seconds to several hours. After development and fixing, protein or nucleic acid bands can be clearly displayed on the X-ray film.
This kit has a unique luminescent/enhanced substrate system, and Western blotting detection of medium fick-grade can be achieved. In addition to X-ray, can also be detected by fluorescent CCD.

Applications:
- Chemiluminescence ELISA - Western Blot - Dot Blot-DNA/RNA - Southern blot-DNA - Northern blot-RNA

Packaging: 10mL / 100mL / 200mL / 500mL

Features:

High sensitivity and high signal-to-noise ratio, it can detect medium fick-grade antigens
The signal lasts for 24 hours, and the luminescence time is more than 10 times longer than that of conventional ECL substrate. High-quality imprinted images can be obtained by multiple exposures for publication
Higher dilution ratio of antibodies can be used to greatly save antibodies
Dilution ratio of primary antibody (liquid storage concentration 1mg/ mL) : 1:1000-1:50000
Dilution ratio of secondary antibody (liquid storage concentration 1mg/ mL): 1:50000-1:250000

Fast Blocking Western - 36122ES
Fast blocking western is a new generation of ready-to-use, efficient, and fast Western blocking solutions. The blocking time is only 10 minutes, which is better than the traditional blocking solutions such as skim milk powder, BSA, and casein, and simplifies the Western blotting experiment. Blocking with Fast blocking western prevents nonspecific binding during antibody detection, but allows specific detection. Compared with blocking solutions, it has a higher signal, lower background, and higher signal-to-noise ratio.The reagent does not contain protein components of mammalian origin to avoid cross reaction with antibodies. This buffer is compatible with phosphorylated antibodies as well as NC and PVDF membranes. But not for biotin labeled antibodies.

Packaging size: 100mL / 500mL

Features:

Ready to use 1x blocking solutions, no need to prepare, easy to use
Short blocking time, only 10 minutes, fast and efficient
Lower background and higher signal-to-noise ratio, better than the traditional blocking solutions such as skim milk pow, der, BSA and casein
It does not contain proteins of mammalian origin, ensures low background and high signal-to-noise ratio, and is compatible with phosphorylated antibodies

Western Blotting