Viral Vector Production - Transfection

Hieff Trans™ PEI Transfection Reagent-GMP - 40821ES
This product is a Linear Polyethylenimine (PEI) after optimization and transformation, with a concentration of 1 mg/mL. This product is purely chemically synthesized and does not contain animal-derived ingredients. It is particularly suitable for the co-transfection of multiple plasmids, the production of recombinant virus vectors, and the instantaneous expression of recombinant proteins. This product has low cytotoxicity, high transfection efficiency, and is compatible with antibiotics. It has high gene expression efficiency in HEK293 and other cells.

This product is produced according to the GMP process requirements and is provided in the form of sterile liquid.

Packaging size: 10 mL / 100 mL

Features:

Small scale and large-scale production of various virus vectors and transient expression of proteins suitable for adherent and suspension systems
High transfection efficiency, less plasmid usage, and high cost performance
Compatible with a variety of commercial medium
There are R&D and GMP products to match the use needs from R&D, process development to large-scale production
Sufficient capacity and stable batch
The audit materials are complete, and the product declaration is not worried

Polyethylenimine Linear(PEI) 40000（rapid lysis) - 40816ES
PEI 40000 is a highly charged cationic polymer with a molecular weight of 40,000 that binds negatively charged nucleic acid molecules very easily, forming a complex and allowing the complex to enter cells. PEI 40000 is a transient transfection reagent with low cytotoxicity, high transfection efficiency, and high gene expression efficiency in cells such as HEK293 and CHO.
PEI 40000 has many advantages over PEI 25000 Transfection Reagent. include:
1. PEI 40000 is easy to dissolve and can be directly dissolved in water. PEI 25000 needs to first adjust the water to weak acid to help it dissolve, and then use NaOH to adjust the pH to neutral.
2. PEI 40000 is easy to operate, easier to use, and has a better transfection effect than PEI 25000; 3. PEI 25000 contains 4-11% propionyl residues, which can prevent the polymer backbone from binding to DNA. Compared to PEI 25000, PEI 40000 is a complete shed construction, so its performance is consistently efficient.
This product is an instant type, which dissolves quickly and is easy to prepare.

Packaging size: 100 mg / 1 g

Features:

Exceptional Efficiency: Superior transfection performance in the broad range of cell lines, including transient transfection and stable transfection
Wide Adaptability: Excellent transfection efficiency in a variety of cell lines and high levels of recombinant protein expression
Low Toxicity: The activities of the diverse cells almost unaffected by transfection reagents from YEASEN
Simple Operation: Proven efficacy in the presence of serum—eliminates the need to change media following transfection
Cost Effective: Competitive transfection effect with more affordable prices

Selected citations:
[1] TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022;185(6):980-994.e15. doi:10.1016/j.cell.2022.02.010(IF:41.584)
[2] Intrinsic Radical Species Scavenging Activities of Tea Polyphenols Nanoparticles Block Pyroptosis in Endotoxin-Induced Sepsis [published correction appears in ACS Nano. 2022 Mar 3;:]. ACS Nano. 2022;16(2):2429-2441. doi:10.1021/acsnano.1c08913(IF:15.881)
[3] The genomic architecture of EBV and infected gastric tissue from precursor lesions to carcinoma. Genome Med. 2021;13(1):146. Published 2021 Sep 7. doi:10.1186/s13073-021-00963-2(IF:11.117)