Transfection

Hieff Trans™ Liposomal Transfection Reagent - 40802ES
Hieff Trans™ Liposomal Transfection Reagent is a versatile liposome transfection reagent, suitable for DNA, RNA, and oligonucleotide transfection, with high transfection efficiency for most eukaryotic cells. Its unique formula allows it to be added directly to the medium, and the presence of serum does not affect transfection efficiency, which reduces the damage to cells caused by serum removal. There is no need to remove the nucleic acid-Hieff Trans™ complex or replace it with a fresh medium after transfection, and it can also be removed after 4-6 hours.
Hieff Trans™ is supplied in sterile liquid form. Usually, for 24-well plate transfection, about 1.5 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections; for a 6-well plate, about 6 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections. 160 transfections.

Packaging size: 0.5 mL / 1 mL / 5×1 mL

Selected citations:
[1] Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins. Nat Biotechnol. 2022 Jan 3. doi: 10.1038/s41587-021-01112-1. PMID: 34980910. (IF:54.908)
[2] CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer. 2021 Feb 2;20(1):25. doi: 10.1186/s12943-021-01321-x. PMID: 33530981; PMCID: PMC7851937. (IF:27.403)
[3] circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c-Myc Translation. Adv Sci (Weinh). 2022 Mar;9(8):e2103817. doi: 10.1002/advs.202103817. Epub 2022 Jan 24. PMID: 35072355; PMCID: PMC8922094. (IF:16.808)
[4] Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication. Clin Transl Med. 2022 Feb;12(2):e684. doi: 10.1002/ctm2.684. PMID: 35184390. (IF:11.492)
[5] A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. J Exp Clin Cancer Res. 2022 Mar 8;41(1):85. doi: 10.1186/s13046-022-02276-7. PMID: 35260179. (IF:11.161)
[6] UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. Neuron. 2021 Jun 16;109(12):1949-1962.e6. doi: 10.1016/j.neuron.2021.04.023. Epub 2021 May 14. PMID: 33991504. (IF:17.17)
[7] Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network. Nucleic Acids Res. 2021 Sep 7;49(15):8556-8572. doi: 10.1093/nar/gkab626. PMID: 34329471; PMCID: PMC8421228.  (IF:16.9)
[8] Loss of Endothelial CXCR7 Impairs Vascular Homeostasis and Cardiac Remodeling After Myocardial Infarction: Implications for Cardiovascular Drug Discovery. Circulation. 2017 Mar 28;135(13):1253-1264. Epub 2017 Feb 2. PMID: 28154007. (IF:18.881)

Features:

Exceptional Efficiency: Superior transfection performance in the broad range of cell lines, including transient transfection and stable transfection
Wide Adaptability: Excellent transfection efficiency in a variety of cell lines and high levels of recombinant protein expression
Low Toxicity: The activities of the diverse cells almost unaffected by transfection reagents from YEASEN
Simple Operation: Proven efficacy in the presence of serum—eliminates the need to change media following transfection
Cost Effective: Competitive transfection effect with more affordable prices

Hieff Trans™ Universal Transfection Reagent - 40808ES
Hieff Trans™ universal transfection reagent is a multi-purpose, convenient and efficient liposome transfection reagent developed based on the latest nanotechnology. It is suitable for the transfection of DNA, RNA and oligonucleotides, including primary cells, Difficult-to-transfect cells, including neurons, have high transfection efficiency. Its unique formula allows it to be added directly to the medium, and the presence of serum does not affect transfection efficiency, which reduces the damage to cells caused by serum removal. It is not necessary to remove the nucleic acid-transfection reagent complex or replace with fresh medium after transfection, and the fresh medium can also be replaced after 4-6 hours according to the nutrient status of the cells.

Packaging size: 0.5 mL / 1 mL / 5×1 mL

Notes:

During the transfection operation, it is better that the cell confluence reaches 70%-90%, and the specific plating density is determined according to the situation of the cells
Preparation of transfection complexes requires dilution of DNA and transfection reagents in serum-free medium
Antibiotics cannot be added to the medium during transfection
The use of high-purity DNA or RNA helps to obtain higher transfection efficiency, and endotoxin in plasmids is the enemy of transfection
The nucleic acid concentration and reagent volume should be optimized for the first use to obtain the maximum transfection efficiency

Hieff Trans™ in vitro siRNA/miRNA Transfection Reagent - 40806ES
Hieff Trans™ in vitro siRNA/miRNA Transfection Reagent is a PEI cationic, amphiphilic transfection reagent developed for the transfection of siRNA into mammalian cells. Suitable for transfection of siRNA and miRNA. This transfection reagent encapsulates siRNA or miRNA to form a cationic complex, which interacts with negatively charged proteoglycans on the cell surface and adsorbs on the cell surface, enters the cell through endocytosis, and forms an inclusion body. The transfection reagent acts as a proton sponge, which can absorb H+ in the lysosome, and the continuous influx of protons causes the complex to swell and rupture, thereby releasing the exogenous siRNA or miRNA into the cytoplasm.

This product can achieve over 90% expression efficiency of 1 nM siRNA in a wide range of cell lines, avoiding off-target effects. Suitable for transfection of a variety of cells, including Hela, MCF-7, HepG2, CHO, and other adherent cells; and difficult to transfect suspension cell lines, such as K562 or THP-1 cells, can achieve 80% silencing efficiency; Including some primary cells, primary human fibroblasts, and primary human hepatocytes, etc., the silencing efficiency of 80% can be achieved.

Packaging size: 0.5 mL / 1 mL

Selected citations:
[1] Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells. Front Cell Dev Biol. 2022;10:829404. Published 2022 Mar 2. doi:10.3389/fcell.2022.829404(IF:6.684)
[2] Resveratrol Treats UVB-Induced Photoaging by Anti-MMP Expression, through Anti-Inflammatory, Antioxidant, and Antiapoptotic Properties, and Treats Photoaging by Upregulating VEGF-B Expression. Oxid Med Cell Longev. 2022;2022:6037303. Published 2022 Jan 4. doi:10.1155/2022/6037303(IF:6.543)
[3] Exosomes Derived From Human Urine-Derived Stem Cells Overexpressing miR-140-5p Alleviate Knee Osteoarthritis Through Downregulation of VEGFA in a Rat Model. Am J Sports Med. 2022;50(4):1088-1105. (IF:6.203)
[4] AKT serine/threonine kinase 2-mediated phosphorylation of fascin threonine 403 regulates esophageal cancer progression. Int J Biochem Cell Biol. 2022;145:106188. (IF:5.085)
[5] Comprehensive transcriptome analysis of hypothalamus reveals genes associated with disorders of sex development in pigs. J Steroid Biochem Mol Biol. 2021;210:105875. (IF:4.294)

Features:

Low toxicity: the transfected cells still maintain good activity
Wide adaptability: ordinary cells and primary cells difficult to transfect are fully covered

Polyethylenimine Linear(PEI) MW 25000 - 40815ES
Linearized Polyethyleneimine PEI 25000 Transfection Reagent is a highly charged cationic polymer that easily binds negatively charged nucleic acid molecules, forms a complex, and allows the complex to enter the cell. The transfection reagent is a transient transfection reagent with low cytotoxicity, high transfection efficiency, and high gene expression efficiency in cells such as HEK293 and CHO. It has been verified that the linear PEI transfection reagent is widely applicable to a variety of cell lines including HEK-293, HEK293T, CHO-K1, COS-1, COS-7, NIH/3T3, Sf9, HepG2 and Hela cells, etc. The reagent is compatible with serum-containing media and can efficiently introduce nucleic acids into cells.

Packaging size: 1 g / 5 x 1g

Selected citations:
[1] Molecular mechanism of agonism and inverse agonism in ghrelin receptor. Nat Commun. 2022;13(1):300. Published 2022 Jan 13. doi:10.1038/s41467-022-27975-9(IF:14.919)
[2] METTL14 promotes prostate tumorigenesis by inhibiting THBS1 via an m6A-YTHDF2-dependent mechanism. Cell Death Discov. 2022;8(1):143. Published 2022 Mar 30. doi:10.1038/s41420-022-00939-0 (IF:5.241)

Notes:

After the prepared PEI solution is taken out from -20 ºC and thawed, it can be stored in a 4 ºC refrigerator and must not be re-frozen.
For most cells, 3.0 μL of PEI transfection reagent per 1 μg of DNA can achieve high transfection efficiency. You can also try to use 1.5~4 μL volume of linear PEI transfection reagent per 1 μg DNA for optimization.

Polyethylenimine Linear (PEI) MW40000, rapid lysis - 40816ES
PEI 40000 is a highly charged cationic polymer with a molecular weight of 40,000 that binds negatively charged nucleic acid molecules very easily, forming a complex and allowing the complex to enter cells. PEI 40000 is a transient transfection reagent with low cytotoxicity, high transfection efficiency, and high gene expression efficiency in cells such as HEK293 and CHO.
PEI 40000 has many advantages over PEI 25000 Transfection Reagent. include:1. PEI 40000 is easy to dissolve and can be directly dissolved in water. PEI 25000 needs to first adjust the water to weak acid to help it dissolve, and then use NaOH to adjust the pH to neutral.2. PEI 40000 is easy to operate, easier to use, and has a better transfection effect than PEI 25000; 3. PEI 25000 contains 4-11% propionyl residues, which can prevent the polymer backbone from binding to DNA. Compared to PEI 25000, PEI 40000 is a complete shed construction, so its performance is consistently efficient.
This product is an instant type, which dissolves quickly and is easy to prepare.
Packaging size: 100 mg / 1 g

Selected citations:
[1] TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022;185(6):980-994.e15. doi:10.1016/j.cell.2022.02.010 (IF:41.584)
[2] Intrinsic Radical Species Scavenging Activities of Tea Polyphenols Nanoparticles Block Pyroptosis in Endotoxin-Induced Sepsis [published correction appears in ACS Nano. 2022 Mar 3;:]. ACS Nano. 2022;16(2):2429-2441. doi:10.1021/acsnano.1c08913(IF:15.881)
[3] The genomic architecture of EBV and infected gastric tissue from precursor lesions to carcinoma. Genome Med. 2021;13(1):146. Published 2021 Sep 7. doi:10.1186/s13073-021-00963-2(IF:11.117)
[4] High-resolution structures of human Na<sub>v</sub>1.7 reveal gating modulation through α-π helical transition of S6<sub>IV</sub>. Cell Rep. 2022;39(4):110735. doi:10.1016/j.celrep.2022.110735(IF:9.423)
[5] Potent and Persistent Antibody Response in COVID-19 Recovered Patients. Front Immunol. 2021;12:659041. Published 2021 May 28. doi:10.3389/fimmu.2021.659041(IF:7.561)

Features:

Exceptional Efficiency: Superior transfection performance in the broad range of cell lines, including transient transfection and stable transfection
Wide Adaptability: Excellent transfection efficiency in a variety of cell lines and high levels of recombinant protein expression
Low Toxicity: The activities of the diverse cells almost unaffected by transfection reagents from YEASEN
Simple Operation: Proven efficacy in the presence of serum—eliminates the need to change media following transfection

Polybrene (hexadimethrine bromide) - 40804ES
Polybrene (hexadimethrine bromide), also known as Polybrene, is a cationic polymer commonly used in DNA transfection experiments with mammalian cells to enhance the transfection efficiency of liposomes. It is widely employed in retrovirus-mediated gene transfection and lentivirus-mediated gene transfection. The mechanism of action may involve neutralizing the sialic acid on the cell surface, facilitating adsorption by overcoming electrostatic repulsion with virus particles. Polybrene is also recognized as an anticoagulant (heparin antagonist) and is frequently used in the production of non-specifically aggregated red blood cells. Additionally, in protein sequencing, low doses of Polybrene have been shown to significantly improve the degradation of peptides in automated sequencing analysis. The addition of Polybrene to PVDF membranes can also enhance membrane affinity.

The product is provided in solution form, and the powder is reconstituted in 0.9% NaCl to make a 10 mg/mL solution, which is then sterilized using a 0.22 μm filter. It is typically diluted at a ratio of 1:1000-1:2000 for use, with specific dilution ratios depending on the cell type, as outlined in relevant literature.