Hieff UNICON™ Hotstart E-Taq DNA Polymerase, 5 U/μL - 10726ES
Hieff UNICON™ Hotstart E-Taq DNA Polymerase features an enhanced hot start Taq DNA polymerase, incorporating dual blocking antibodies. Both the 5'→3' polymerase and 5'→3' exonuclease activities of Taq DNA polymerase are effectively inhibited. A 30-second heating step at the pre-denaturation temperature serves to completely deactivate the antibodies, restoring DNA polymerase and exonuclease activities. This dual blocking capability not only safeguards against nonspecific amplification due to mismatch or primer dimer but also proficiently restrains the reduction in fluorescence signal caused by probe degradation. This ensures enhanced stability of the in vitro detection reagent during transportation or room temperature usage.
Packaging size: 250 U / 1000 U / 10000 U
Manual
Features: Applications:
- High sensitivity
- effectively inhibit the non-specific amplification
- Routine PCR amplification of DNA fragments
- Hotstart PCR
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Hieff™ Double-Block anti-Taq DNA Polymerase Antibody (20U/μL) - 31303ES
Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody is a mixture of two monoclonal antibodies who can inhibit the activity of 5 '- 3' polymerase and 5 '- 3' exonuclease of Taq DNA Polymerase, respectively. The antibody can effectively inhibit the nonspecific annealing of primers and the nonspecific amplification. In addition, the product can effectively prevent probe degradation. Hieff™ Double-Block anti-Taq DNA Polymerase Antibody is denatured in the initial DNA denaturation step of PCR reaction, through which the activity of DNA polymerase is restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibodies.
A total of 1 μL antibody could block the activity of 20 U of Taq DNA polymerase. It is recommended to mix the antibody and the Taq DNA polymerase at room temperature for 1 hour (incubate at room temperature for 2 hours when volume is greater than 200 mL, and customer should adjust the process when applied to larger volume), and then store at -20℃ overnight before use.
Note: The specific activity of different Taq DNA Polymerase is variant, the blocking ratio needs to be adjusted appropriately to achieve that the blocking efficiency is better than 95%. Packaging size: 100 μg (400U) / 1 mg (4KU) / 5mg (20KU) Manual
Note: The specific activity of different Taq DNA Polymerase is variant, the blocking ratio needs to be adjusted appropriately to achieve that the blocking efficiency is better than 95%. Packaging size: 100 μg (400U) / 1 mg (4KU) / 5mg (20KU) Manual
Applications:
- Hot-start PCR
- Compatible with Taq mutants
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Hifair™ V Reverse Transcriptase - 11300ES
Hifair™ V Reverse Transcriptase is an updated version of HieffTM M-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability, and reaction temperature limit (up to 60°C) than HieffTM M-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. HifairTM V Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes.
Packaging size: 10000 U / 5x10000 U
Manual
Features: Applications:
- High-temperature resistance: Suitable for RNA templates with complex secondary structures.
- Suitable for reverse transcription of a small number of templates and low-copy genes.
- Quantification of fluorescence
- Cloning of genes
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Murine RNase inhibitor (40 U/μL) - 10603ES
Murine RNase Inhibitor is purified from a recombinant strain of E. coli in a soluble form. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1:1 ratio with high affinity. Recombinant murine RNase inhibitor does NOT contain 2 oxidation-sensitive cysteine which are contained in human-origin RNase inhibitor. Therefore, murine RNase inhibitor has high anti-oxidation activity and is more stable for low DTT experiments (< 1mmol/L). This product is validated for its compatibility with HifairTM Ⅱ Reverse Transcriptase (Cat#11110) and various DNA Polymerases. Murine RNase inhibitor is ideal for high-DTT-sensitive experiments, such as RT-PCR. The product can be used in cDNA Synthesis, polysome isolation and In vitro transcription/translation.
Packaging size: 2 KU / 10 KU / 20 KU / 100 KU
Manual
Features:
- Performs under a wide range of reaction conditions
- Protects RNA from degradation at temperatures up to 55°C
Selected citations:
[1] A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction. Biomaterials. 2022;285:121541. doi:10.1016/j.biomaterials.2022.121541(IF:12.479)
[2] RBM39 Alters Phosphorylation of c-Jun and Binds to Viral RNA to Promote PRRSV Proliferation. Front Immunol. 2021;12:664417. Published 2021 May 17. doi:10.3389/fimmu.2021.664417(IF:7.561)
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RNase HII (2 U/μL) - 14539ES
RNase HII is a ribonuclease internal enzyme obtained from the Pyrococcus abyssi and recombinantly expressed in E. Coli. It recognizes the DNA-rN-DNA/DNA double strand, cutting at the site where ribonucleotides are incorporated into DNA. However, its activity on single-stranded RNA is very low, and it shows no cutting activity on dsDNA or ssDNA. RNase HII cuts at the site of a single ribonucleotide residue from the 5' end, producing a 5' phosphate group and a 3' hydroxyl end after cutting. This RNase HII enzyme has optimal activity at 70~75°C, is active between 50°C and 75°C, but has very low activity at room temperature. The product is highly thermostable, with almost no loss of activity after incubation at 95°C for 45 minutes, and is compatible with various PCR reaction systems.
Packaging size: 250 U
Manual
Applications:
- Detection by LAMP with high-sensitivity probes
- RNase HII-dependent PCR (rhPCR)
- Removal of mismatched ribonucleotides formed during polymerase chain reaction
- Degradation of the RNA portion of Okazaki fragments
Uracil DNA Glycosylase (UDG/UNG), 1 U/μL - 14455ES
UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP.
Packaging size: 100 U / 500 U / 10000 U
Manual
Features:
- 10 U of this product was detected by E.coli 16S rDNA-specific TaqMan qPCR, and the result showed that E.coli genome residue was fewer than 10 copies
- No nucleic acid endonuclease, exonuclease and RNase residues
- Uracil is the sole base recognized by this enzyme
- Remove ssDNA and dsDNA, but they are inactive to RNA
- Eliminate false positive results due to contamination of PCR amplification products.
- Compatible with PCR, qPCR, RT-qPCR, etc
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