Protein Quantification

BCA Protein Quantification Kit BCA - 20201ES
BCA (Bicinchoninic acid) method is a widely used method for protein concentration determination at present. Based on the biuret reaction, that is, in an alkaline environment, the protein reduces Cu2+ to Cu+, producing a purple blue complex, which has a high absorbance value at 562 nm. The amount of the reaction product is proportional to the protein concentration. BCA protein concentration determination method is simple, sensitive, fast and stable. The protein standard provided in the kit provides convenience for users to make standard curves.The BCA protein concentration determination kit can be used for colorimetric assay and microplate assay. The former requires a large amount (100 μL) of protein sample, The ratio of protein sample to BCA working solution is 1:20 (v/v), which reduces the impact of interfering substances. The latter is simple and convenient to operate, requiring only a small amount (10-25 μL) of protein sample. However, because the ratio of protein sample to BCA working solution is 1:8 (v/v), the tolerance concentration of interfering substances is limited to some extent and the minimum detection level is reduced. Our company provides three sizes of BCA protein concentration detection kits, the colorimetric method can be used for 50 times, 250 times and 500 times respectively. The enzyme labeling method can be used for 500 times, 2500 times and 5000 times respectively.

Packaging size: 500T (100mL) / 2500T (500mL) / 5000T (1000mL)

Features:

High sensitivity, with a minimum detection protein of 0.2 μg. The lower limit of detection concentration reaches 10 μg/mL.
It is faster and takes less time to develop color than the general BCA protein concentration determination kit. It is about 4 times faster than the traditional Lowry method
Wide linear range, there is a good linear range in the concentration range of 20-2000 μg/mL
It is not affected by the chemicals in most of the samples

Bradford Protein Quantification Kit - 20202ES
Bradford method is one of the most sensitive methods used to determine protein concentration. It is based on the Bradford dye (Coomassie brilliant blue G250) and protein combination, the maximum absorption peak of dye from A456 to A595, and the determination of the absorption value and protein concentration is proportional to the principle of design. The method can calculate the protein concentration by the light absorption value and realize the rapidity and simplicity of protein concentration determination. High sensitivity, about four times higher than Lowry method, the lowest protein detection can reach 1 μg. The determination is fast and simple, requiring only one reagent, and is not affected by the chemical reagents in most samples.Our company provides two sizes of Bradford protein concentration detection kits, the colorimetric method can be used 125 times, and 625 times respectively. The enzyme labeling method can be used 500 times and 2500 times respectively.

Packaging size: 500T (125mL) / 2500T (625mL)

Features:

Single reagent, no working reagent preparation required
Fast—almost immediate color development; add, mix, and read results