Protein Electrophoresis

Precast Protein Plus Gel, 4-20%, 10 wells, Hepes-Tris, 10 well - 36250ES
Precast Protein Plus Gel series adopt HEPES-Tris buffer system, which has the advantage of excellent separation effect and greatly shortens the electrophoresis time. The Precast Protein Gel plate is made of plastic, and the special perfusion technology can ensure the stability and consistency of strip distribution between batches of Precast Protein Gel, and the electrophoresis effect is stable. The height of the stacking gel is 1.5 cm, the gradient concentration is 4-12% and 4-20%, and the fixed concentration is 8%, 10%, 12%, and 15%. The number of sample loading wells of each concentration gel is 10 and 15, respectively. The maximum sample loading is 70 μL for 10 wells, and 40 μL for 15 wells. This series of gels do not contain SDS and can be used for both natural and denatured protein electrophoresis. When used for natural protein electrophoresis, only the protein with an isoelectric point less than 7 can be separated. The electrophoresis solution must be matched with the electrophoresis solution. 

Packaging size: 10 gels (1 box)

Features:

Easy to use: ready to use; Tear off the adhesive tape from the bottom of the plastic board
Wide range of applications: compatible with denatured protein and natural protein
Clear band: the special treatment of plastic plates greatly reduces protein adsorption
Time-saving: the experiment can be completed in 18 minutes at the fastest
Quality assurance: strict inter-batch stability assurance
Long shelf life: can be stored at 2℃ ~ 8℃ for one year

Precast Protein Plus Gel, 4-20%, 15 wells, Hepes-Tris, 15 well - 36256ES
Precast Protein Plus Gel series adopt HEPES-Tris buffer system, which has the advantage of excellent separation effect and greatly shortens the electrophoresis time. The Precast Protein Gel plate is made of plastic, and the special perfusion technology can ensure the stability and consistency of strip distribution between batches of Precast Protein Gel, and the electrophoresis effect is stable. The height of the stacking gel is 1.5 cm, the gradient concentration is 4-12% and 4-20%, and the fixed concentration is 8%, 10%, 12%, and 15%. The number of sample loading wells of each concentration gel is 10 and 15, respectively. The maximum sample loading is 70 μL for 10 wells, and 40 μL for 15 wells. This series of gels do not contain SDS and can be used for both natural and denatured protein electrophoresis. When used for natural protein electrophoresis, only the protein with an isoelectric point less than 7 can be separated. The electrophoresis solution must be matched with the electrophoresis solution. 

Packaging size: 10 gels (1 box)

Features:

Easy to use: ready to use; Tear off the adhesive tape from the bottom of the plastic board
Wide range of applications: compatible with denatured protein and natural protein
Clear band: the special treatment of plastic plates greatly reduces protein adsorption
Time-saving: the experiment can be completed in 18 minutes at the fastest
Quality assurance: strict inter-batch stability assurance
Long shelf life: can be stored at 2℃ ~ 8℃ for one year

MiniPro™ PET 4 Vertical Electrophoresis Cell, 4-gels, for 1.0 mm thick gels - 80211ES
MiniPro™ PET 4 Vertical Electrophoresis Cell is used to run pre-casting gel and hand-casting gel, at most 4 pieces of gels are available simultaneously. The Electrophoresis Chamber is compatible with 1-D and 2-D electrophoresis application. The gel-casting frame and spacer glass plate make the activity of hand-casting gel simple and avoid the leakage.

Components:

Spacer glass plate: It is the relatively high glass plate with fastening side strip. There are 3 kinds of glass plate with the thickness of 0.75mm, 1.0mm, 1.5mm respectively
Short glass plate: It is the relatively short glass plate and spacer glass plate to form the gel sandwich clamp
Gel-casting frame: It is put on the desk and align the short glass plate with spacer glass plate, and make sure to form the gel sandwich clamp
Components of gel clamp: 1 piece of gel casting frame, a piece of spacer glass plate and a piece of short glass plate
Gel base: Pressure leverage grip the components of gel to sealing gasket closely to avoid the leakage of gel
Gel sandwich: It consists of short glass plate and spacer glass plate as well as polymer gel
Replacement plate for single gel: Transparent mound pressing baffle, it is used to cast 1 piece of gel or 3 pieces of gels
Electrophoresis core: It is used to hold the gel sandwich and supply the U-sealing strip and upper and lower electrode as well as connecting plug. Anode is marked by red while the cathode is marked by black

Gold Band Plus 3-color Regular Range Protein Marker (8-180 kDa) - 20350ES
This product consists of 10 highly purified and prestained proteins ranging in molecular weight from 8 kDa to 180 kDa (8,17,25,33,43,55,72,100,130,180kDa), among them, 72 kDa is orange band, 8 kDa is green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents!After the prestained protein is combined with the dye, the molecular weight will change in the gel of different concentrations. There are signs in the instructions, this product is only for reference when judging the molecular weight of the target protein.

Applications:
- Protein migration is monitored during SDS-PAGE electrophoresis- Monitor protein transfer to the membrane after Western blotting- Protein size analysis on SDS-PAGE and Western blotting

Packaging size: 250μL / 2× 250μL / 10× 250μL

Selected citation:
The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal. NPJ Biofilms Microbiomes. 2022;8(1):16. Published 2022 Apr 4. doi:10.1038/s41522-022-00280-5(IF:7.290)

Features:

Bright color - three color predye, each strip average concentration of 2.5 μg
Lower amount of this product into the gel hole - mini-gel: 3-5 μL; 
Stable performance - 50℃ for 20 h, no degradation
Long shelf life - can be stored at -15℃ ~ -25℃ for two years

GoldBand Plus 3-color High Range Protein Marker (25-300 KDa) - 20347ES
This product consists of 9 highly purified and prestained proteins ranging in molecular weight from 25 kDa to 300 kDa (25,45,72,100,130,160,200,250,300 kDa), among them, 72 kDa is an orange band, 25 kDa is a green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to the o PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents! After the prestained protein is combined with the dye, in different buffer systems, there is displacement. There are signs in the instructions, this product is only for reference when judging the molecular weight of the target protein.

Applications:- Protein migration is monitored during SDS-PAGE electrophoresis- Monitor protein transfer to the membrane after Western blotting- Protein size analysis on SDS-PAGE and Western blotting

Packaging size: 250μL / 2×250μL / 10×250μL

Features:

Bright color - three color predye, each strip average concentration of 2.5 μg
Lower amount of this product into the gel hole - usually used for loading 5-10 μL each time
Stable performance - 50℃ for 20 h, no degradation
Long shelf life - can be stored at -30℃ ~ -15℃ for two years

Precast Running Buffer for Native PAGE, 2 L (Powder) - 36258ES
This buffer is specially designed for the high resolution precast gel (Cat#36245-Cat#36256), for the high resolution precast gel (Hepes-Tris), this product has a better electrophoresis effect, effective for natural protein electrophoresis. The product is provided in powder form. Before use, ddH2O can be fixed to 200 mL and prepared into 10×mother liquor, and then diluted into 2 L in the proportion of 10 times solution; Or directly add 2 L water, with 1× solution for use.

Packaging size: 2L/bag

Features:

Easy to use: Open the wrapping paper, pour out the powder, and add ddH2O to 2 L in a constant volume
Formula: 2 L 1×HEPES running buffer: Tris 12.112 g, HEPES 23.832 g, EDTA 1.17 g
Long shelf life: can be stored at room temperature for two years

Precast Running Buffer for SDS PAGE, 2 L (Powder) - 36257ES
This buffer is specially designed for the high resolution precast gel (Cat#36245-Cat#36256), for the high resolution precast gel (Hepes-Tris), this product has a better electrophoresis effect, and is effective for denatured protein electrophoresis. The product is provided in powder form. Before use, ddH2O can be fixed to 200 mL and prepared into 10×mother liquor, and then diluted into 2 L in the proportion of 10 times solution; Or directly add 2 L water, with 1×solution for use.

Packaging size: 2L/bag

Features:

Easy to use: Open the wrapping paper, pour out the powder, and add ddH2O to 2 L in a constant volume
Long shelf life: can be stored at room temperature for two years
Formula: 2 L 1×HEPES running buffer: Tris 12.112 g, HEPES 23.832 g, SDS 2 g, EDTA 1.17 g

Commassie Blue Fast Stain Solution (8 mins) - 20309ES
The Coomassie brilliant blue staining method is often used in the laboratory to stain the isolated proteins on the gel. It not only overcomes the limitation of the low sensitivity of amino black staining, but also is easier to operate than silver staining. Coomassie brilliant blue is widely available in G250 and R250. Coomassie brilliant blue G250 is often used in the determination of protein content because of its rapid binding reaction with protein. Coomassie brilliant blue R250 reacts slowly with proteins, but can be eluted off, so it can be used to stain electrophoresis bands.This product is designed based on the Coomassie brilliant blue staining method for protein staining. Compared with traditional stain solution, this product is a new type of PAGE dyeing reagent, which does not contain toxic or irritating substances such as methanol and acetic acid. Its unique formula enables the function of surfactant stripping, protein fixation, protein staining, shielding non-protein area coloring at the same time. 8-10 minutes to finish PAGE protein staining without decolorization. The resolution of dyeing is equivalent to that of traditional staining methods. The stained protein bands or protein spots can be used for high efficiency electroelution and are suitable for protein spectrum analysis.

Packaging size: 8ml (125× concentrate)

Features:

Does not contain toxic or irritating substances such as methanol and acetic acid
8-10 minutes to finish PAGE protein staining without decolorization
Rinsed several times with clean water on a rotating shaker, the residual weak background can be completely removed