Biopharma - Plasmid Linearization

BspQI GMP-grade (10 U/μL) - 10664ES
This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.

Features:                                                                             Applications:

Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Restriction Enzyme Cut Site: GCTCTTC (1/4)
Digestion of DNA to obtain specific sticky ends
Isolated from a recombinant source
Tested for the absence of endonucleases, exonucleases, RNases

Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G)
Linearize plasmid template before in vitro transcription
Restriction digest

FuniCut™ BsaI - 15005ES
FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps and can be used to digest plasmid, genomic and viral DNA as well as PCR products.

Features:                                                                             Applications:

Rapid digestion: DNA was digested rapidly by an enzyme for 5-15 min
Universal buffer: one tube reaction, simplifying the process
Stringent quality control

Molecular cloning
Southern blotting
SNP
Genotyping

FuniCut™ XbaI - 15033ES
FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps and can be used to digest plasmid, genomic and viral DNA as well as PCR products.

Features:                                                                             Applications:

Rapid digestion: DNA was digested rapidly by an enzyme for 5-15 min
Universal buffer: one tube reaction, simplifying the process
Stringent quality control

Molecular cloning
Southern blotting
SNP
Genotyping