Viral Vector Production - Nuclease

UCF.ME™ UltraNuclease GMP-grade (250 U/μL) - 20157ES
UltraNuclease is a genetically engineered endonuclease from Serratia marcescens. The enzyme digests all forms of DNA/RNA moleculars into oligonucleotides of about 5 bp. The product is expressed in Escherichia coli (E. coli) and purified under the GMP environments. The nuclease is equivlent to benzonase in reducing host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing.  It can also used in protein study to reduce the viscosity of cell supernatant and cell lysate, increase protein purification efficiency and enhance protein functional research.
UltraNuclease is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.

Packaging size: 25 KU / 50 KU / 100 KU / 1 MU / 5 MU

Features:                                                                             Applications:

Wide range of applications: degrade all forms of DNA and RNA 
High purity and activity: purity ≥ 99%; specific activity ≥ 1.5×106 U/mg
Strong adaptability: strong tolerance, adapt to a variety of reaction conditions
Comply with pharmacopeia: no animal origin; no antibiotics; lower endotoxin
Qualification: product gets the FDA DMF filling which can shorten the customers for new drug application
GMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufacture

Purification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses, removing DNA/RNA from proteins and other biologicals
Reduction of viscosity caused by nucleic acids
Sample preparation in electrophoresis and chromatography
Prevention of cell clumping

Salt Active UltraNuclease GMP-grade - 20159ES
Salt Active UltraNuclease is a nonspecific endonuclease and has optimum activity at high salt concentration. Salt can play an important role in minimizing aggregation of protein or virus, which would be helpful for dissociation of DNA & RNA from proteins or other cellular components. In high salt conditions, enzymes are more likely to access the released DNA & RNA and degrade them. Nuclease which has high activity at high salt would be more effective to improve purification processes. The Salt Active UltraNuclease can be used to reduce the viscosity of cell supernatant and cell lysate, increase purification efficiency in high salt condition.
The enzyme can also reduce host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing. 

Packaging size: 25 KU / 100 KU / 1 MU / 5 MU

Features:

High purity (≥ 98%)
Animal-free production
Low Endotoxin
High activity at high salt conditions
Supported by ELISA Kit