Hieff NGS™ Dual Barcode Fast-Pace DNA Cyclization Kit for MGI - 13340ES
The Hieff NGS™ Dual Barcode Fast-Pace DNA Cyclization Kit for MGI is a single-strand cyclization kit specifically designed for MGI platforms. The use of high-quality enzymes and optimized Buffer significantly improve reaction efficiency, enabling the entire cyclization and digestion process to be completed in less than 30 minutes. This kit is suitable for all standard dual-barcode libraries for MGI platforms and is not limited to different MGI sequencing platforms except for the limitations of the library-prep reagents.

Packaging size: 16 T / 96 T

Features:

Wide range of use: compatible with mainstream library preparation kits (KAPA, etc.)
Fast cyclization: a 1-hour rapid cyclization
High cyclization efficiency: the cfDNA cyclization efficiency>30%, other sample types>40%

Hieff NGS™ Fast-Pace DNA Cyclization Kit for MGI - 13341ES
Hieff NGS™ Fast-Pace DNA Cyclization Kit for MGI is a single-strand cyclization kit specifically designed for MGI platforms. The use of high-quality enzymes and optimized Buffer significantly reaction efficiency, enabling the entire cyclization and digestion process to be completed in less than 30 minutes. This kit is suitable for all standard single-barcode libraries for MGI platforms and is not limited to different MGI sequencing platforms except for the limitations of the library-prep reagents.

Packaging size: 16 T / 96 T

Wide range of use: compatible with mainstream library preparation kits (KAPA, etc.)
Fast cyclization: a 1-hour rapid cyclization
High cyclization efficiency: the cfDNA cyclization efficiency>30%,other sample types>40%

Hieff NGS™ Novel DNA Ligation Module for kit - 12805ES
Hieff NGS™ Novel DNA ligation module for kit is a DNA ligation module designed for Illumina® & MGI®high-throughput sequencing platform. This kit concludes the novel high-efficiency T4 DNA ligase independently developed by Yeasen. Hieff NGS™ Novel DNA ligation module for kit is optimized for use with the Hieff NGS™ Ultima Endprep mix (Cat#12605) or the Hieff NGS™ OnePot Pro DNA Fragmentation Reagent (Cat#12619). All reagent components provided in this product have undergone strict quality and function verification to ensure the excellent performance and inter batch stability of the product to the highest extent.

Packaging size: 24 T / 96 T

Hieff NGS™ OnePot Pro DNA Fragmentation Module (end repair and dA-tailing plus) - 12619ES
Hieff NGS™ OnePot Pro DNA Fragmentation Module provides a new-generation enzymatic fragmentation reagent  designed for Illumina® and MGI® high-throughput sequencing platforms . This product simplifies the operation process compared with the complex mechanical interruption and also greatly reduces the time and cost by performing fragmentation, end repair and dA-tailing of dsDNA in one reaction.  The fragmentation products can immediately for adaptor ligation with no cleanup steps by using Hieff NGS™ Fast-Pace DNA Ligation Module (Cat#12607) or Hieff NGS™ Novel DNA Ligation Module (Cat#12626). Hieff NGS™ OnePot Pro DNA Fragmentation Module can work with a broad range of DNA inputs (animal, plant and microbial genomes), ranging from 500 pg-1μg in library preparation. 

Packaging size: 24 T / 96 T

Notes:

Compatible with a range of 500 pg - 1 μg input DNA. High quality input DNA (A260/A280 = 1.8-2.0) is highly recommended.
High concentration of metal ion chelator or other salt remained in Input DNA may affect subsequent experiments, it is recommended to dilute DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
For conventional high-quality genomic DNA, fragmentation time refers to Table 1. This product is compatible with various samples with different GC content and has minimal bias. For the FFPE samples with different degrees of degradation, the fragmentation time refers to Table 2. Customers need to fine-tune the recommended fragmentation time in their own experimental system to achieve the best results.
The DNA can be digested to the required size according to the recommended fragmentation time. In order to ensure the high-quality and stable fragmentation effect, the fragmentation process should be operated on ice.

Hieff NGS™ Ultima Endprep Mix - 12605ES
Hieff NGS™ Ultima Endprep mix is a directional optimized end repair and dA tailing module designed for Illumina® & MGI®high throughput sequencing platform. The end of 500 pg - 1 μg DNA fragment can be repaired efficiently with this kit. The 5' end of the repair product contains phosphate group and the 3' end has dA protrusion. Compared with the conventional library construction method, Hieff NGS™ Ultima Endprep Mix adopts one-step reaction process, which combines the End Repair and dA tailing steps, and meanwhile reduces the reaction time of each step, thus significantly reducing the experiment time. Hieff NGS™ Ultima Endprep mixis optimized for use with the Hieff NGS™ Novel DNA ligation module for kit (Cat#12805). All reagent components provided in this product have undergone strict quality and function verification to ensure the excellent performance and inter batch stability of the product to the highest extent.

Packaging size: 24 T / 96 T

NGS Module