NGS Enzyme

Phi29 DNA polymerase (10 U/µL)- 14404ES
Phi29 DNA polymerase, derived from Bacillus subtilis phage, has been genetically engineered to exhibit excellent strand displacement and continuous synthesis capabilities, enabling the synthesis of DNA fragments up to 70 kb. Additionally, it has strong 3'→5' exonuclease proofreading activity, thus it is recommended to modify the 3' end of primers in the system to prevent degradation. It is commonly used for in vitro plasmid synthesis and whole genome synthesis.

Packaging size: 250 U / 1000 U / 5000 U
Unit definition: The amount of enzyme required to incorporate 0.5 pmol of dNTP into acid-insoluble precipitate in 10 minutes at 30°C.

UCF.ME™ High Affinity RNase Inhibitor (40 U/μL) - 14675ES
UCF.ME™  High Affinity RNase Inhibitor (40 U/μL) is a recombinant RNase inhibitor expressed in E. coli. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1:1 ratio with high affinity.
The host nucleic acid residue of UCF.ME™ High Affinity RNase Inhibitor (40 U/μL) is lower with the purification process specially developed by YEASEN, which is suitable for application with more stringent requirements on background bacteria, such as pathogen microorganism detection.
Packaging size: 2 KU / 20 KU / 100 KU
Unit definition: The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2', 3'-CMP to generate 3'-CMP.

Applications:

Single-Cell Library Preparation
RT-qPCR

Quick T4 DNA Ligase (400 U/μL) - 10301ES
Quick T4 DNA Ligase is a single enzyme product that can be used for the ligation of DNA fragments and adapters in the process of NGS library construction. This product has been verified by high-throughput sequencing and has excellent quality. For customers who do not need experimental system adjustment, it is recommended to purchase Hieff NGSTM Fast-Pace DNA Ligation Module (Cat#12607).

Packaging size: 40 KU / 400 KU
Unit definition: In a 20 μL ligation reaction system, when 6 μg of λDNA-Hind Ⅲ is reacted at 16℃ for 30 mins, the amount of enzyme required to give more than 50% ligation of the DNA fragments was defined as one unit (U).

Hieff™ Versatile T4 DNA Ligase (600 U/µL) Thermotolerant and High Fidelity - 12996ES
Hieff™ Versatility T4 DNA Ligase is a variant of T4 DNA Ligase. This enzyme is suitable for catalyzing the ligation reaction between cohesive or blunt-ended molecules. It is a single-enzyme product designed for connecting DNA fragments and adapters during the library preparation process in next-generation sequencing(NGS). The Hieff® Versatility T4 DNA Ligase has been extensively validated through high-throughput sequencing and exhibits exceptional quality. It is high fidelity and thermotolerant.

Packaging size: 60 KU / 600 KU
Unit definition: In a ligation reaction system of 20 μL, the enzyme amount required to catalyze the ligation of more than 50% of DNA fragments, when 6 μg of λDNA-Hind III is reacted at 16°C for 30 minutes, is defined as 1 unit of activity (U).

Specifications:

Source: Recombinant E.coli
Storage Buffer: 50 mM Tris-HC1,100 mM KC1,0.1 mM EDTA,1 mM DTT,50%Glycerol,pH 7.5士0.2 @25℃

Hifair™ IV Reverse Transcriptase - 11112ES
Hifair™  IV Reverse Transcriptase is an updated version of Hifair™ Ⅱ Reverse Transcriptase obtained through genetic engineering technology. It has higher cDNA synthesis ability and speed, thermal stability and reaction temperature limit (up to 60°C) than Hifair™ Ⅱ Reverse Transcriptase. The synthesized cDNA product is up to 19.8 kb. Hifair™ IV Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structure or low copy genes.

Packaging size: 10000 U / 5x10000 U
Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using Oligo(dT) as primers.

2×Super Canace™ II High-Fidelity Mix for Library Amplification - 12621ES
2×Super Canace™ Ⅱ High-Fidelity Mix for Library Amplification is a ready-to-use 2×pre-mixed solution. Including high-fidelity DNA Polymerase (6 times the Fidelity of ordinary pfu DNA polymerase, amplification speed of 15 sec/kb), dNTP and optimized buffer system for high-throughput sequencing library amplification. This mix offers advantages such as quick and easy operation, high sensitivity, strong specificity, and good stability. During library amplification, the system only requires the addition of primers and templates, simplifying the experimental steps, reducing the human error, and improving experimental throughput and result reproducibility. Additionally, the mix contains specific protective agents that maintain stable activity even after repeated freeze-thaw cycles.
This product has been validated for DNA library construction in conjunction with Hieff NGS™ Fast-Pace End Repair/dA-Tailing Module (Cat#12608) and Hieff NGS® Fast-Pace DNA Ligation Module (Cat#12607), Its effectiveness has been verified through sequencing on Illumina high-throughput platform. All reagent components provided in this product undergo rigorous quality control to ensure excellent performance and batch-to-batch stability.

Packaging size: 24 T / 96 T

T4 Polynucleotide kinase(10 U/μL) - 12902ES
T4 Polynucleotide Kinase (T4 PNK) is a polynucleotide 5'-hydroxyl kinase that catalyzes the transfer of the γ-phosphate from ATP to the 5'-OH group of oligonucleotide chains (double- or single-stranded DNAs or RNAs) and nucleoside 3'-monophosphates, and the reaction is reversible. Has 3'- phosphatase activity, hydrolyzes the 3'-phosphate group from the 3'-phosphate end of oligonucleotides, deoxy3'-monophosphate nucleosides and deoxy3'-diphosphate nucleosides. In the presence of ADP, T4 PNK has 5'- phosphatase activity, catalyzing the exchange of 5'-P-oligo/polynucleotide and ATP terminal 5'-phosphate groups. It is suitable for DNA library construction and probes end labeling.

Packaging size: 500 U / 2500 U / 10000 U
Unit definition: The required amount of T4 Polynucleotide Kinase to catalyze the recombination reaction of 1 nmol [γ-32P]-ATP in 30 min at 37 °C.

Applications:
Phosphorylation of DNA or RNA 5´-end for ligation
End-labeling of DNA or RNA for use as probes and DNA sequencing
5'-phosphorylation of oligonucleotides that has been phosphorylated at the 3´-end to prepare a pNp substrate for addition to the 3´ end of DNA or RNA
Labeling 5'-termini of oligonucleotides with a 3'-phosphate group

T4 DNA polymerase (5 U/μL) - 12901ES
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'-3' direction and requires the presence of templates and primers, and T4 DNA Polymerase has 3'→5' exonuclease activity but does not have 5'→3' exonuclease activity.

Packaging size: 100 U / 500 U / 2000 U
Unit definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into polynucleotides in 30 minutes at 37°C.
* Also available as T4 DNA Polymerase (25 U/μL) - 14451ES, 25 KU / 125 KU

Applications:
Catalyze the blunt ends of the 5' or 3' protruding ends of DNA
Labeled DNA probe synthesis by a displacement reaction
Synthesis of the second chain during fixed-point mutation
Cloning of PCR products that do not depend on ligation reactions

RNase H (5 U/μL) - 12906ES
RNase H (ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA on hybrid DNA/RNA strands, but will not hydrolyze phosphates in single- and double-stranded DNA or RNA diester bonds.

Packaging size: 100 U / 500 U / 5000 U
Definition on enzymatic activity: In 50 μL reaction system, the amount of enzyme required to hydrolyze the RNA-DNA hybrid strand to generate 1 nmol of ribonucleotides was defined as 1 U at 37°C for 20 min.

Applications:
Remove mRNA before cDNA double-strand synthesis
In RT-PCR/RT-qPCR experiments, remove RNA after the synthesis of one strand of cDNA
Remove poly(A) after Oligo(dT) hybridizes with mRNA
Cleavage of RNA-specific sites

Hieff™ Novel T4 DNA Ligase (400 U/µL) - 10298ES
Hieff™ Novel T4 DNA Ligase is a single enzyme product that can be used for DNA fragments and adapters ligation during NGS library preparation. The product has been verified by high-throughput sequencing and has excellent quality. This enzyme has efficient ligation ability and is compatible with various samples.

Packaging size: 40 KU / 400 KU
Unit definition: The amount of enzyme required to catalyze the ligation of more than 50% of the DNA fragments in a 20 μL ligation reaction system is defined as 1 activity unit (U ) when 6 μg of λDNA Hind III catabolite is reacted at 16 °C for 30 min.

Hieff NGS™ S-Taq DNA Polymerase for dA-Tailing- 13486ES
Hieff NGS™  S-Taq DNA Polymerase for dA-Tailing is a new Taq DNA Polymerase designed for Illumina®or MGI® high-throughput sequencing platforms. The enzyme has 5'→3' polymerase activity and 5'→3' exonucleation enzyme activity, no 3'→5' exonuclease activity. It has been verified that the enzyme can be stably and efficiently applied to the NGS library preparation process of dA-Tailing step at 3' end, compatible with the conventional end repair and dA-Tailing system, and can also be used for customer's current dA-Tailing system.

Packaging size: 24 T / 96 T / 1000 T

Hieff Canace™ Uracil+ High-Fidelity DNA polymerase (1 U/μL) - 10145ES
Hieff Canace™ Uracil+ High-Fidelity DNA polymerase, is a new generation of high-fidelity DNA Polymerase based on Pfu DNA Polymerase. The Hieff Canace™ Uracil+ high-fidelity DNA Polymerase enables rapid and accurate PCR reactions for complex templates. Its fidelity is improved significantly, and it completely avoids the amplification failure caused by using dUTP-containing primers/templates/dNTPs. The product is equipped with an optimized enzyme buffer and the addition of PCR-enhancing components, making the enzyme highly efficient and adaptable to a wide range of templates, and suitable for amplification of complex templates.

Useful for high-fidelity library amplification using dUTP-containing primers/templates/dNTPs.

Hieff Canace™ Uracil+ High-Fidelity DNA polymerase (1 U/μL) -10145ES

Packaging size: 100 U / 500 U
* Also available as Hieff Canace™ Uracil+ High-Fidelity DNA polymerase (3 U/μL) -14384ES, 1000 T / 5000 T

Fast T4 DNA ligase (400 U/μL) - 10299ES
Fast T4 DNA Ligase is a single enzyme product that can be used to connect DNA fragments and adapter in the process of NGS library construction. The product has been verified by high throughput sequencing and has excellent quality. This enzyme has a highly efficient ligating ability, which is very suitable for the ligating of complex nucleic acid fragments, such as the Ω adapter of the MGI platform. It is recommended to select Hieff NGSTM Ultima DNA Ligation Module (Cat#12604) whose core component is Fast T4 DNA Ligase for customers who don’t need system adjustment.

Packaging size: 40 KU / 400 KU
Unit definition: In a 20 μL ligation reaction system, when 6 μg of λDNA-Hind Ⅲ is reacted at 16℃ for 30 mins, the amount of enzyme required to give more than 50% ligation of the DNA fragments was defined as one unit (U).

DNA Polymerase I (10 U/μL) - 12903ES
DNA Polymerase I, in the presence of template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'—3' direction. The molecular weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'—3' exonuclease activity and single-strand-specific 3'—5 exonuclease activity. This enzyme can be used with DNase I (Cat#10607) for nick translation reaction and with RNase H (Cat#12906) to synthesize the second strand of cDNA.

Packaging size: 500 U / 2500 U / 10000 U