Mycoplasma Detection
GMyc-PCR Mycoplasma Test Kit - 40601ES
Cell culture is a common experiment in life science research. Unlike other commonly used experimental methods, cell culture is a dynamic continuous process, and cells often respond to manipulation errors or contaminants that often exhibit abnormal cell states or medium appearance. If it is contaminated by mycoplasma, the cell morphology has no obvious change, and it is easy to be overlooked. It is often not found until the pollution is very serious. There may be hundreds of mycoplasmas on the contaminated cell membrane, these mycoplasmas compete for nutrients and release toxic metabolites, seriously affecting the experimental results.
Studies have shown that at least 20 kinds of mycoplasma can contaminate cells, among which the most common are: oral Mycoplasma (M. orale), Mycoplasma arginine (M. arginini), Mycoplasma hyorhinis (M. hyorhinis), Mycoplasma fermentum (M. fermentans), Mycoplasma hominis (M. hominis), Mycoplasma salivarius (M. salivarium), Mycoplasma pulmonary (M. pulmonis) and Mycoplasma pear (M. pirum). The mycoplasma contamination rate of cultured cells ranges from 4% to 92%. The sources of contamination include the working environment, the operator itself (some mycoplasmas are normal flora of the human body), culture medium, serum, cell cross-contamination, experimental equipment, and used Contamination of the original tissue or organ from which cells were prepared.
Identifying the underlying cause of problems during cell culture is a difficult and time-consuming task, where any sudden changes should be suspected, and good testing practices and regular testing for mycoplasma contamination are necessary. There are many methods for the detection of mycoplasma, such as direct culture, DNA fluorescence staining, ELISA, and PCR methods.
GMyc-PCR Mycoplasma Detection Kit mainly uses the PCR method to detect Mycoplasma infection of various biological materials (such as cell culture, experimental animal secretions, animal serum, etc.). It combines several advantages: sensitive, specific, rapid, and can be detected directly with cell culture supernatants. This product detects mycoplasma in biological materials such as cultured cells by PCR method. The primers used are designed according to the conserved region of the 16S-23S rRNA sequence of mycoplasma, and only specifically amplify the mycoplasma DNA, with high detection sensitivity and specificity. PCR amplification and electrophoresis analysis only take a few hours, and the operation is convenient and simple. Packaging size: 10 T / 20 T
Studies have shown that at least 20 kinds of mycoplasma can contaminate cells, among which the most common are: oral Mycoplasma (M. orale), Mycoplasma arginine (M. arginini), Mycoplasma hyorhinis (M. hyorhinis), Mycoplasma fermentum (M. fermentans), Mycoplasma hominis (M. hominis), Mycoplasma salivarius (M. salivarium), Mycoplasma pulmonary (M. pulmonis) and Mycoplasma pear (M. pirum). The mycoplasma contamination rate of cultured cells ranges from 4% to 92%. The sources of contamination include the working environment, the operator itself (some mycoplasmas are normal flora of the human body), culture medium, serum, cell cross-contamination, experimental equipment, and used Contamination of the original tissue or organ from which cells were prepared.
Identifying the underlying cause of problems during cell culture is a difficult and time-consuming task, where any sudden changes should be suspected, and good testing practices and regular testing for mycoplasma contamination are necessary. There are many methods for the detection of mycoplasma, such as direct culture, DNA fluorescence staining, ELISA, and PCR methods.
GMyc-PCR Mycoplasma Detection Kit mainly uses the PCR method to detect Mycoplasma infection of various biological materials (such as cell culture, experimental animal secretions, animal serum, etc.). It combines several advantages: sensitive, specific, rapid, and can be detected directly with cell culture supernatants. This product detects mycoplasma in biological materials such as cultured cells by PCR method. The primers used are designed according to the conserved region of the 16S-23S rRNA sequence of mycoplasma, and only specifically amplify the mycoplasma DNA, with high detection sensitivity and specificity. PCR amplification and electrophoresis analysis only take a few hours, and the operation is convenient and simple. Packaging size: 10 T / 20 T
Features:
- The primers used were designed according to the conserved region of Mycoplasma 16S-23S rRNA sequence
- Only amplified mycoplasma DNA specifically
- Excellent sensitivity and specificity
- More than 20 mycoplasma species could be detected
Selected citations:[1] AMPK-mediated phosphorylation enhances the auto-inhibition of TBC1D17 to promote Rab5-dependent glucose uptake. Cell Death Differ. 2021;28(12):3214-3234. doi:10.1038/s41418-021-00809-9(IF:15.828)
[2] Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells. Cell Res. 2017;27(8):967-988. doi:10.1038/cr.2017.82(IF:15.606)
[3] Nuclear translocation of p85β promotes tumorigenesis of PIK3CA helical domain mutant cancer. Nat Commun. 2022;13(1):1974. Published 2022 Apr 13. doi:10.1038/s41467-022-29585-x(IF:14.919)
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[4] Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA. Nat Chem Biol. 2018;14(7):680-687. doi:10.1038/s41589-018-0065-9(IF:13.843)
[5] Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome. Nat Chem Biol. 2016;12(5):311-316. doi:10.1038/nchembio.2040(IF:12.709)
[6] 2-Hydroxylation of Fatty Acids Represses Colorectal Tumorigenesis and Metastasis via the YAP Transcriptional Axis. Cancer Res. 2021;81(2):289-302. doi:10.1158/0008-5472.CAN-20-1517(IF:12.701)
MycGuard™ Plus-Color One-Step Mycoplasma Detection Kit - 40612ES
The MycAwayTM Plus-Color One-Step Mycoplasma Detection Kit is a rapid detection product for mycoplasma contamination in cell culture which was developed based on the unique isothermal amplification technology of YEASEN. Compared with the previous mycoplasma detection products, this kit is optimized and has greatly reduced the false positive rate, improved the accuracy of the test, and enhanced the identification of negative and positive results. At the end of amplification, the sample can be left at room temperature for a period of time without the change from negative to positive, and thus will not affect the judgment of the results.
The principle is that if the cell culture is contaminated with mycoplasma, the conserved sequences of mycoplasma DNA will be amplified heavily and rapidly, causing the color of the reaction solution to change from blue-purple to sky-blue, and the result is visible to the naked eye, without electrophoresis.
MycAwayTM Plus-Color One-Step Mycoplasma Detection Kit can detect multiple mycoplasma species, including eight that are common in cell culture. The traditional nested PCR method for mycoplasma detection is susceptible to influence by the inhibitors in cell culture supernatant and showed a false-negative result. Also if needed to perform the electrophoresis, cap opening may increase the contamination and lead to a false-positive result. The one-step mycoplasma detection kit has none of these disadvantages, also its detection sensitivity and accuracy are much higher than those of the PCR method. Packaging size: 25 T / 100 T
The principle is that if the cell culture is contaminated with mycoplasma, the conserved sequences of mycoplasma DNA will be amplified heavily and rapidly, causing the color of the reaction solution to change from blue-purple to sky-blue, and the result is visible to the naked eye, without electrophoresis.
MycAwayTM Plus-Color One-Step Mycoplasma Detection Kit can detect multiple mycoplasma species, including eight that are common in cell culture. The traditional nested PCR method for mycoplasma detection is susceptible to influence by the inhibitors in cell culture supernatant and showed a false-negative result. Also if needed to perform the electrophoresis, cap opening may increase the contamination and lead to a false-positive result. The one-step mycoplasma detection kit has none of these disadvantages, also its detection sensitivity and accuracy are much higher than those of the PCR method. Packaging size: 25 T / 100 T
Features:
- The one-step operation results in less than 60 minutes
- Use a water bath or PCR instrument to test
- 27 mycoplasma species were accurately detected by the kit, including 8 common species
- Results just identified by the visual inspection
Selected citations:[1] Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids. Nat Biomed Eng. 2022;6(2):195-206. doi:10.1038/s41551-021-00774-1(IF:25.671)[2] Neuroendocrine Regulation of Stress-Induced T Cell Dysfunction during Lung Cancer Immunosurveillance via the Kisspeptin/GPR54 Signaling Pathway. Adv Sci (Weinh). 2022;9(13):e2104132. doi:10.1002/advs.202104132(IF:16.806)[3] Transcription-coupled structural dynamics of topologically associating domains regulate replication origin efficiency. Genome Biol. 2021;22(1):206. Published 2021 Jul 12. doi:10.1186/s13059-021-02424-w(IF:13.583)
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[4] FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape. Theranostics. 2022;12(2):842-858. Published 2022 Jan 1. doi:10.7150/thno.66575(IF:11.556)[5] Inhibition of Rspo-Lgr4 Facilitates Checkpoint Blockade Therapy by Switching Macrophage Polarization. Cancer Res. 2018;78(17):4929-4942. doi:10.1158/0008-5472.CAN-18-0152(IF:9.130)
Mycoplasma Elimination
MycAway™ Treatment (1000×) - Mycoplasma Elimination Reagent - 40607ES
In the process of cell cultures, regular detection can effectively avoid mycoplasma infection, but if the cells have been infected with mycoplasma, they need to be treated in time. The best treatment is to discard the cells after high-pressure sterilization to avoid contaminating other clean cell lines. If contaminated cells are precious, mycoplasma infection must be removed. Because mycoplasma lacks the cell wall, conventional antibiotics are generally ineffective. This product is an improved mixed preparation specially used for mycoplasma elimination. It can achieve a good mycoplasma elimination effect by inhibiting the synthesis of related proteins and is not harmful to cells. Minimize the loss of your precious cells due to mycoplasma infection.
Packaging size: 100 μL / 1 mL / 5 ×1 mL
Features:
- Ultra-low Toxicity: Only block the bacterial protein synthesis rather than animal cells
- Excellent Stability: Stored at -15℃ ~ -25℃ for 18 months
- Facilitated Operation: Just added into the culture medium
- Rapid Onset: Take effect in 3 days
- Wide Applicability: Effective against most mycoplasmas
Selected citations:
[1] Parallel profiling of antigenicity alteration and immune escape of SARS-CoV-2 Omicron and other variants. Signal Transduct Target Ther. 2022;7(1):42. Published 2022 Feb 8. doi:10.1038/s41392-022-00910-6(IF:18.187)
[2] Dynamic methylome of internal mRNA N<sup>7</sup>-methylguanosine and its regulatory role in translation. Cell Res. 2019;29(11):927-941. doi:10.1038/s41422-019-0230-z(IF:17.848)
[3] Ambient particulate matter triggers dysfunction of subcellular structures and endothelial cell apoptosis through disruption of redox equilibrium and calcium homeostasis [published correction appears in J Hazard Mater. 2021 Jan 15;402:123316]. J Hazard Mater. 2020;394:122439. doi:10.1016/j.jhazmat.2020.122439(IF:9.038)
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Mycoplasma Prevention
MycoFog Biodecontamination Starter Kit - MF-100
The MycoFog™ system is an easy to use, hands free method of disinfecting tissue culture isolators and incubators, especially for Mycoplasma sp. contamination. MycoFog™ is a valuable tool for laboratories that need to maintain a clean and contaminant-free environment. The instrument is particularly useful for laboratories that work with tissue- and cell-culture controlled environment equipment including isolators, incubators, glove boxes, and anaerobic or hypoxic chambers.
Items included:- MycoFog™ Biodecontamination Fogger- 2 MycoFog™ Reagent Pods- Manual- Additional Wick- USB Cable
MycoFog Prove-It-To-Yourself Kit - MF-200
Items included:- MycoFog™ Biodecontamination Fogger- 2 MycoFog™ Reagent Pods- Manual- Additional Wick- USB Cable- 4 G. stearothermopphilus biological indicators
MycoFog Fogger - MF-300
Eliminates Biocontamination From Laboratory Incubators.
The Biodecontamination Fogger is a battery-powered piezo-driven nebulizing instrument that creates the hydrogen peroxide fog that biodecontaminates your incubator.
By regularly using MycoFog™, you can help to prevent the spread of Mycoplasma and other contaminants in your lab and protect your cell cultures. Labs that need a fast, simple, and cost-effective way to decontaminate their cell culture incubators will find our product an indispensable adjunct to their contamination control protocol.
By regularly using MycoFog™, you can help to prevent the spread of Mycoplasma and other contaminants in your lab and protect your cell cultures. Labs that need a fast, simple, and cost-effective way to decontaminate their cell culture incubators will find our product an indispensable adjunct to their contamination control protocol.
Reagent Pods 18ml (4 units) - MF-400
The Biodecontamination Reagents are a measured dose of 7.8% hydrogen peroxide in a light- and air- sealed easy-open container for biodecontamination of isolators incubators and closed workstations . This is poured into the reagent reservoir of the MycoFog™ Biodecontamination Fogger for use in isolators and incubators up to 200L.
For use in isolators and incubators up to 500L, Catalog #MF-500 MycoFog Reagent 25ml
MycGuard™ Solution (500×), for Disinfecting Ordinary Water Bath - 40610ES
Mycoplasma contamination often occurs during the cell culture process, but as it’s too small and difficult to be detected by the naked eye and microscopic equipment, so the mycoplasma contamination is often difficult to be found when it occurs. This undoubtedly increases the difficulty of the mycoplasma detection and removal, so it is particularly important to do a good job in the prevention of mycoplasma.
Not only the materials or places such as cell culture plate, culture reagent, super clean table or biosafety cabinet are easy to be contaminated by the mycoplasma, the water dish in the cell incubator and water bath is also the potential environment that may cause mycoplasma pollution. In order to protect your cells from the influence of mycoplasma, in addition to providing the direct removal reagents for mycoplasma, YEASEN also provided the Mycoplasma prophylaxis reagents to prevent mycoplasma contamination of normal cells, which included preventive reagent (directly added into the cell culture medium), preventive spray, preventive reagent for water dish in cell incubator and water bath (directly added in proportion).
This product is a mycoplasma preventive reagent for water bath. It is provided in the form of 500× concentrated solution. It is recommended to clean the water bath first and then add the diluted (1×) preventive reagent. Packaging size: 100 mL
Not only the materials or places such as cell culture plate, culture reagent, super clean table or biosafety cabinet are easy to be contaminated by the mycoplasma, the water dish in the cell incubator and water bath is also the potential environment that may cause mycoplasma pollution. In order to protect your cells from the influence of mycoplasma, in addition to providing the direct removal reagents for mycoplasma, YEASEN also provided the Mycoplasma prophylaxis reagents to prevent mycoplasma contamination of normal cells, which included preventive reagent (directly added into the cell culture medium), preventive spray, preventive reagent for water dish in cell incubator and water bath (directly added in proportion).
This product is a mycoplasma preventive reagent for water bath. It is provided in the form of 500× concentrated solution. It is recommended to clean the water bath first and then add the diluted (1×) preventive reagent. Packaging size: 100 mL
- Mycoplasma prevention in the water bath
- Broad spectrum activity
- Effective at low concentration
MycGuard™ Solution (100×), for Disinfecting Water Bath of CO2 Incubator - 40609ES
Mycoplasma contamination not only involves places that directly contact cells such as cell culture plates, culture reagents, or ultra-clean benches but also equipment such as water trays and water baths in cell culture incubators that may cause mycoplasma contamination. In addition to providing scavenging agents that directly remove mycoplasma agents, normal cells are also provided with mycoplasma preventive agents to prevent mycoplasma contamination. It mainly includes the following aspects: reagent prevention (directly added to the cell culture medium), ultra-clean bench prevention (spray), water bath prevention (directly added to the water bath) and cell incubator water tray prevention, etc.
This product is a mycoplasma prevention reagent in the water plate of the cell incubator. It is provided in the form of a 100× concentrated solution. It can be directly added to the water plate according to the proportion when using it. It is recommended to replace it every 2~4 weeks. Packaging size: 100 mL Selected citation: [1] AMPK-mediated phosphorylation enhances the auto-inhibition of TBC1D17 to promote Rab5-dependent glucose uptake. Cell Death Differ. 2021;28(12):3214-3234. doi:10.1038/s41418-021-00809-9(IF:15.828)
This product is a mycoplasma prevention reagent in the water plate of the cell incubator. It is provided in the form of a 100× concentrated solution. It can be directly added to the water plate according to the proportion when using it. It is recommended to replace it every 2~4 weeks. Packaging size: 100 mL Selected citation: [1] AMPK-mediated phosphorylation enhances the auto-inhibition of TBC1D17 to promote Rab5-dependent glucose uptake. Cell Death Differ. 2021;28(12):3214-3234. doi:10.1038/s41418-021-00809-9(IF:15.828)
