High Fidelity PCR
2× Hieff Canace™ AdvanceFast High-Fidelity PCR Master Mix (With Dye) - 10164ES
Ready-to-use 2× pre-mixed solution containing Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase, dNTPs, and an optimized buffer system, which contains pre-added electrophoresis indicators. The pre-mix contains pre-added electrophoresis indicator, PCR products can be directly electrophoresed, the amplification products are flat ends. 2× Hieff Canace™ AdvanceFast PCR Master Mix (With Dye) has the advantages of quick and easy, high sensitivity, high specificity, good stability, etc., the reaction system can be added with only the primers and templates. In addition, the product also contains a specific protective agent, so that the premix can still maintain stable activity after repeated freezing and thawing.
Figure: Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has high fidelity compared to other brands' products (A). The assay was done by examining the mutation frequency of PCR products of 6 GC-rich (about 80%) segments amplified from the genomes of different species. The PCR products amplified by Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase were cloned into vectors, and 100 selected single clones for each sequence were subjected to Sanger sequencing.
Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has high amplification speed (B), high GC amplification compatibility (C), and blood tolerance (D). Results from customers' PCR and sequencing show that Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has good performance in PCR yields and specificity (E-H).
Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has high amplification speed (B), high GC amplification compatibility (C), and blood tolerance (D). Results from customers' PCR and sequencing show that Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has good performance in PCR yields and specificity (E-H).
Features:
- Gene cloning
- Amplification of complex templates DNA
- High-throughput library building.
Hieff Canace™ Plus High-Fidelity DNA Polymerase - 10153ES
Hieff Canace™ Plus High-Fidelity DNA Polymerase is based on the Pyroccus Furiosis DNA Polymerase, genetically engineered. The enzyme has a 5'→3' DNA polymerase activity and a 3'→5' exonuclease activity, and its fidelity is 83 times that of Taq DNA polymerase and 9 times that of ordinary Pfu DNA polymerase. Two monoclonal antibodies at room temperature that inhibit polymerase activity and 3'→5' exonuclease activity were added to the enzyme solution, which can easily perform highly specific Hot Start PCR, greatly improving the detection rate of amplification and the specificity of the product. The addition of an extension factor to the enzyme solution gives the enzyme the ability to amplify long fragments, and the length of the amplification of the fragment of interest can be up to 13 kb. This product is equipped with an optima buffer that makes the enzyme suitable for amplification of complex templates. It generates blunt ends in the amplification products.
Applications: QC:
- Using the human genome as a template, a fragment with a GC content of 72% was amplified using Taq DNA Polymerase.
- Strictly to ensure no detection of exonuclease or endonuclease residue
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