Library Preparation
DNA Library Preparation
Hieff NGS™ OnePot Pro DNA Library Prep Kit V3 (Enzymatic) - 12194ES
Hieff NGSTM OnePot Pro DNA Library Prep Kit V3 is a new generation enzymatic fragmentation-based library prep kitspecially developed and designed for Illumina &MGI sequencing platform. Compared to traditional library construction methods, this product employs high-quality fragmentation enzymes, eliminating the cumbersome ultrasonic process. It simplifies the operation by combining the fragmentation and end-repair modules into one. Additionally, the enzymes and buffer for the ligation module are pre-mixed, significantly reducing the time and cost of library construction. This makes it more suitable for automated library construction.. This library prep kit has an excellent library conversion rate and is applicable for samples from all common animals, plants, microorganisms, etc., and also the FFPE samples. On the basis of the previous generation of library construction kit, this product exhibits higher efficiency in fragmentation, end repair, dA-tailing, and adapter ligation than the previous versions. The high-fidelity enzyme significantly improves the uniformity and fidelity of amplification.
Packaging size: 8 T / 24 T / 96 T
Features: Applications:
- Convenient, ready-to-use mix
- Thermostable—half-life is more than 40 min at 95℃
- Generates PCR products with 3'-dA overhangs
- Routine PCR amplification of DNA fragments
- High throughput PCR
- DNA labeling
Hieff NGS™ DNA Library Prep Kit (Mechanical) - 13577ES
Hieff NGSTM DNA Library Prep Kit is a new generation library construction kit specially developed and designed for Illumina® &MGI® sequencing platform. On the basis of the previous generation of library construction kit, this product exhibits higher efficiency in end repair, dA-tailing, and adapter ligation than the previous versions. The high-fidelity enzyme significantly improves the uniformity and fidelity of amplification. The kit is compatible with most DNA sample types, including standard genomic DNA from animals/plants/microorganisms, FFPE samples, cfDNA, and ChIP DNA.
Packaging size: 8 T / 24 T / 96 T
RNA Library Preparation
Hieff NGS™ EvoMax RNA Library Prep Kit (Strand-specific) - 12340ES
Hieff NGSTM EvoMax RNA Library Prep Kit (dUTP) is a premixed, actinomycin D free and strand-specific total RNA sequencing library prep kit compatible with Illumina and MGI platforms. This product is available in two types: tube or sealing kit, and this kit is more convenient whether by Automated Liquid Handling Device or manual manipulation . The kit contains RNA fragmentation reagents, reverse transcription reagents, strand-specific ds-cDNA synthesis reagents, and library amplification reagents. It can be linked with the mRNA purification kit or the rRNA removal kit to do mRNA or lncRNA research. This product optimizes the reverse transcription module to obtain a high chain specificity library without actinomycin D, which ensures the safety of the experimenters to a greater extent. All reagents were subjected to strict quality control and functional validation to maximize the stability and repeatability of the library preparation.
Packaging size: 24 T / 96 T / 96 T (automation) / 96 T (plate)
Figures on FFPE RNA samples: RNA Library prep for different quality FFPE samples. For severely degraded FFPE RNA (DV 200 <50%) and low input samples, we recommend a Double-purification protocol after adapter ligation to reduce library loss. Peak patterns of different quality FFPE samples
.JPG@webp)
.JPG@webp)
.JPG@webp)
.JPG@webp)
.JPG@webp)
Hieff NGS™ Ultima Dual-mode RNA Library Prep Kit - 12308ES
Hieff NGS™ Ultima Dual-mode RNA Library Prep Kit is a total RNA sequencing library construction kit for the Illumina® and MGI® sequencing platform, including RNA fragmentation reagents, reverse transcription reagents, conventional and strand-specific ds-cDNA synthesis reagents, and library amplification reagents. The sequencing library can be constructed followed by the mRNA purification kit or rRNA removal kit. The two-strand synthesis module is equipped with two buffers to meet the need for a conventional library or strand-specific library. Among them, dTTP is replaced with dUTP in the strand-specific two-strand synthesis Buffer, so dUTP can be added to the second strand of cDNA. The high-fidelity DNA polymerase used in this kit cannot amplify the DNA template containing uracil, achieving strand specificity. All reagents provided have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.
Packaging size: 24 T / 96 T
Note: This kit is compatible with both Illumina and MGI platforms, but additional Illumina or MGI Primer Mix ( Cat# 13335 Primer Mix for Illumina and Cat# 13334 Primer Mix for MGI ) is required.
Selected citations:
[1] Deciphering the gut microbiome of grass carp through multi-omics approach. Microbiome, 2024 IF:19.4
[2] Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition. Cancer Research, 2024 IF:12.7
[3] Klotho-derived peptide 1 inhibits cellular senescence in the fibrotic kidney by restoring Klotho expression via posttranscriptional regulation. Theranostics, 2024 IF:12.4
[4] Exoskeleton Partial‐Coated Stem Cells for Infarcted Myocardium Restoring. Advanced Materials, 2023 IF: 29.4
[5] Genomic innovation and regulatory rewiring during evolution of the cotton genus Gossypium. Nature Genetics, 2022 IF:41.4
Hieff NGS™ DNA&RNA Library Co-Prep Kit V2 - 12305ES
Hieff NGS® DNA&RNA Library Co-Prep Kit V2 is a DNA&RNA co-library kit for Illumina®&MGI® sequencing platform, which contains efficient cDNA synthesis reagent and enzyme digestion reagent. Compared with the traditional library construction method, this product can efficiently complete cDNA synthesis and DNA&RNA library construction in one tube.The kit contains high quality enzyme for DNA fragmentation and combines DNA fragmentation, end-repair, and dA-tailing into one step, which reduce time significantly and cost of library preparation. This library prep kit has an excellent library conversion rate and is applicable for samples from all common animals, plants, microorganisms, etc., and also the FFPE samples. This upgraded kit using the latest optimized ligase greatly decreases the self-ligation rate during adapter ligation. Moreover, the introduction of a new high-fidelity polymerase further improves the homogeneity and fidelity of amplification.
All the components provided by the kit have undergone strict quality control and functional verification, which ensures the stability and repeatability of library construction to the greatest extent. Packaging size: 8T / 24 T / 96 T Note: The kit is compatible with dual platforms of Illumina®&MGI®, but additional primer mix (CAT # 13334 Primer Mix for MGI® and Cat# 13335 Primer Mix for Illumina®) is required.
All the components provided by the kit have undergone strict quality control and functional verification, which ensures the stability and repeatability of library construction to the greatest extent. Packaging size: 8T / 24 T / 96 T Note: The kit is compatible with dual platforms of Illumina®&MGI®, but additional primer mix (CAT # 13334 Primer Mix for MGI® and Cat# 13335 Primer Mix for Illumina®) is required.
.JPG@webp)
.JPG@webp)
.JPG@webp)
rRNA Depletion
Hieff NGS™ MaxUp Human rRNA Depletion Kit (Human/Mouse/Rat) - 12257ES
Hieff NGS™ MaxUp Human rRNA Depletion Kit (rRNA & ITS/ETS) is designed to remove rRNA and 45S ITS/ETS from human, mouse and rat total RNA based on RNase H-based workflow and to retain mRNA and other non-coding RNA. This kit is suitable for both intact and partially degraded RNA samples (e.g., FFPE RNA). Since degraded FFPE samples usually contain a higher proportion of ITS/ETS than fresh tissue samples, this kit adds probes in the Human 45S ITS/ETS region, and ITS removal can significantly increase the proportion of valid data in raw data.
Packaging size: 8T / 24 T / 96 T
Applications:
- Gene Expression Research- Alternative splicing analysis- Detection and discovery of non coding RNA- Identification of selective polyadenylation sites- Fusion gene detection
Selected citation:
[1] CRISPR-iPAS: a novel dCAS13-based method for alternative polyadenylation interference. Nucleic Acids Res. 2022;50(5):e26. doi:10.1093/nar/gkac108(IF:16.971)
Features:
- Strong specificity: specifically remove rRNA and ITS/ETS from human samples, especially for FFPE samples
- High compatibility of template starting amount: applicable to 100 ng~1μg sample
- High removal effect: for rRNA, ITS and ETS in human\mouse and rat samples, the removal effect is more than 95%
- Stable quality: strict batch performance and stability quality control
Hieff NGS™ MaxUp rRNA Depletion Kit (Plant) - 12254ES
Hieff NGS™ MaxUp rRNA Depletion Kit (Plant) is designed to remove rRNA (including cytoplasmic 18S and 25S rRNA, mitochondrial 18S and 26S rRNA, chloroplast 16S and 23S rRNA) from plant total RNA based on RNase H-based workflow and to retain mRNA and other non-coding RNA. This kit is suitable for both intact and partially degraded RNA samples.
Packaging size: 24 T / 96 T
Specifications:
- Depletion Technology: RNase H
- Sample Type: Total RNA of Plant
- Final Product Type: mRNA and other non coding RNAs
- Starting Material Amount: 100 ng~1 μg total RNA
- Target: Remove rRNA
Features:
- Strong specificity: specifically remove rRNA from various plant samples
- High compatibility of template starting amount: applicable to 100 ng~1μg sample
- Stable quality: strict batch performance and stability quality control
Globin mRNA Depletion Probe (Human) - 12806ES
Globin mRNA Depletion Probe (Human) is designed to remove human Globin mRNA. It can effectively remove Globin mRNA from adult, infant and embryonic sources, including HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ. This product is used with Hieff NGSTM MaxUp rRNA Depletion Kit (Human/Mouse/Rat) (Yeasen#12253) to effectively remove rRNA and Globin mRNA from total RNA. This kit is suitable for both intact and degraded RNA samples. The RNA samples after rRNA & Globin mRNA removal can be used for high-throughput sequencing analysis of mRNA and non-coding RNA, which significantly enhance the proportion of valid data in sequencing results, and cDNA synthesis or other downstream applications.
Suitable for 100 ng~1 μg total RNA samples human, mouse and rat blood resource, and for both intact or partially degraded RNA samples.
.JPG@webp)
