IP & CO-IP

Protein A/G MagBeads (IP Grade) - 36417ES
Protein A/G MagBeads use "nano-surface biotechnology" (S-TEC) to target Protein A/G onto the surface of superparamagnetic polymer microspheres with high density. It has higher antibody binding ability and very low non-specific adsorption rate of protein. One-step purification can isolate the antibody with purity >90% from the serum sample, which is simple and effective to use. Natural Protein A is a cell wall surface protein found in Staphylococcus aureus. Natural Protein G is a cell surface protein isolated from the genus G or C Streptococcus.  Both have similar functions and bind most mammalian IgG by interacting primarily with the Fc region of immunoglobulin (Ig), but differ in their binding specificity. Protein A/G MagBeads covalently conjugate both protein A and protein G, providing A wider binding range and higher utility than either protein A or protein G alone. At the same time, this product uses genetically modified proteins A and G, which not only maintain their Ig affinity properties, but also remove the non-major binding domain of the natural protein itself to reduce non-specific binding. This product has a wide range of application, can be used in cell lysis fluid, cell secretory fluid supernatant, serum, animal ascites and other immune antigen samples.

Applications:
- Immunoprecipitation (IP)- Purification of antibody

Selected citations:
[1] Precise targeting of osteopontin in non-small cell lung cancer spinal metastasis to promote chemosensitivity via a smart hollow nano-platform. Chemical Engineering Journal. 2022;436:132131.(IF:15.1)
[2] lncRNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5. Mol Ther. 2022;30(1):448-467. (IF:11.454)
[3] FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination. Clinical and Translational Medicine. 2022;12(7):e891.(IF:10.6)

Pakaging size: 1mL / 5mL

Features:

Low background—little to no nonspecific binding, and no preclearing
Highly reproducible—uniform beads ensure the most consistent results
Highly sensitive—Magbeads technology is the most cited method for sensitive applications, such as ChIP and IP,  of low abundance proteins
Fast and easy— no centrifugation or preclearing steps
Versatile usage for IP, Co-IP, pull-down, and ChIP assays

V5 Tag Peptide - 20575ES
V5 Tag Peptide is a synthetic peptide. This is a 14-amino acid polypeptide isolated from the P and V proteins of simian virus 5 (SV5). It is used to competitively bind anti-V5 antibodies in immunoassay assay. The binding of V5 Tag peptides to antibodies is specific; therefore, V5 Tag peptides are a mild, ideal reagent for elution of V5 fusion proteins bound to anti-V5 monoclonal antibodies. The recommended working concentration of V5 Tag peptides for elution of V5-Tag fusion proteins is 100-400 μg/mL. 

Packaging size: 5mg / 25mg

Features:                                                                             Applications:

High stability
High purity

Purify V5 fusion proteins
Competitively bind Anti-V5 monoclonal antibodies in immunoassay assay

HA Tag Peptide - 20574ES
HA Tag Peptide is a synthetic peptide. The amino acid sequence is YPYDVPDYA. The erythrocyte lectin surface antigen determinant derived from influenza virus is identical with the HA amino acid sequence in the HA-Tag fusion protein. Easy to detect by Anti-HA antibody and ELISA. It is used to competitively bind anti-HA antibodies in immunoassay assay. The binding of HA Tag peptides to antibodies is specific; therefore, HA Tag peptides are a mild, ideal reagent for elution of HA fusion proteins bound to anti-HA monoclonal antibodies. The recommended working concentration of HA Tag peptides for elution of HA-Tag fusion proteins is 100-400 μg/mL. 

Packaging size: 5mg / 25mg

Features:                                                                             Applications:

High stability
High purity

Purify HA fusion proteins
Competitively bind Anti-HA monoclonal antibodies in immunoassay assay

c-Myc Tag Peptide - 20573ES
c-Myc Tag peptide is a synthetic polypeptide. Its amino acid sequence corresponds to the 410-419 sequence of the C-terminal of human c-Myc protein. c-Myc Tag peptides are substitutes for c-Myc fusion proteins. It is used to competitively bind anti-c-Myc antibodies in immunoassay assay. The binding of c-Myc Tag peptides to antibodies is specific; therefore, c-Myc Tag peptides are a mild, ideal reagent for elution of Myc-tag fusion proteins bound to anti-Myc monoclonal antibodies.

Packaging size: 5mg / 25mg

Features:                                                                             Applications:

High stability
High purity

Purify c-Myc fusion proteins
Competitively bind Anti-c-Myc monoclonal antibodies in immunoassay assay

Anti-V5 Affinity Gel - 20588ES
Anti-V5 Affinity Gel is prepared by conjugating high quality murine IgG2b monoclonal antibody with Sepharose 4B gel. This product has a high V5 label fusion protein loading capacity (at least 0.5 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with V5 labels.

Packaging size: 1mL / 5mL / 25mL / 100mL

Features:                                                                             Applications:

Specific—highly specific monoclonal antibody
High yield and high purity
Versatile—It can be used for protein purification or COIP
Convenient—reagents and peptides to elute are available separately

Immunoprecipitation (IP)
Purification of fusion proteins with V5 labels

Anti-Myc Affinity Gel - 20587ES
Anti-Myc Affinity Gel is prepared by conjugating high quality murine IgG1 monoclonal antibody with Sepharose 4B gel. This product has a high Myc label fusion protein loading capacity (at least 0.9 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with Myc labels.

Packaging size: 1mL / 5mL / 25mL / 100mL

Features:                                                                             Applications:

Specific—highly specific monoclonal antibody
High yield and high purity
Versatile—It can be used for protein purification or COIP
Convenient—reagents and peptides to elute are available separately

Immunoprecipitation (IP)
Purification of fusion proteins with Myc labels

Anti-His Affinity Gel - 20589ES
Anti-His Affinity Gel is prepared by conjugating high quality murine IgG1 monoclonal antibody with Sepharose 4B gel. This product has a high His label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with His labels.

Packaging size: 1mL / 5mL / 25mL / 100mL

Features:                                                                             Applications:

Specific—highly specific monoclonal antibody
High yield and high purity
Versatile—It can be used for protein purification or COIP
Convenient—reagents and peptides to elute are available separately

Immunoprecipitation (IP)
Purification of fusion proteins with His labels

Anti-HA Affinity Gel - 20586ES
Anti-HA Affinity Gel is prepared by conjugating high quality murine IgG1 monoclonal antibody with Sepharose 4B gel. This product has a high HA label fusion protein loading capacity (at least 1.5 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with HA labels.

Packaging size: 1mL / 5mL / 25mL / 100mL

Features:                                                                             Applications:

Specific—highly specific monoclonal antibody
High yield and high purity
Versatile—It can be used for protein purification or COIP
Convenient—reagents and peptides to elute are available separately

Immunoprecipitation (IP)
Purification of fusion proteins with HA labels

Anti-GFP MagBeads - 20564ES
Anti-GFP MagBeads are magnetic polymer beads. They are made of high quality murine monoclonal antibodies and carboxyl magnetic beads. They provide rapid magnetic responsiveness. Superparamagnetism, good dispersion, uniform particle size, very low non-specific binding, and abundant binding sites. 

Applications: IP, Co-IP, pull-down, and ChIP assays

Packaging size: 500 μL / 1 mL / 5 mL

Features:

Low background—little to no nonspecific binding, and no preclearing
Highly reproducible—uniform beads ensure the most consistent results
Highly sensitive—Magbeads technology is the most cited method for sensitive applications, such as ChIP and IP,  of low abundance proteins
Fast and easy— no centrifugation or preclearing steps

Anti-DYKDDDDK (Flag) MagBeads - 20565ES
Anti-DYKDDDDK (Flag) MagBeads are magnetic polymer beads. They are made of high quality murine monoclonal antibodies and carboxyl magnetic beads. They provide rapid magnetic responsiveness. Superparamagnetism, good dispersion, uniform particle size, very low non-specific binding and abundant binding sites. Anti-DYKDDDDK (Flag) MagBeads can bind to the Met modified N-flag fusion Protein (MET-flag-Protein), N-flag fusion protein (Flag-protein), C-terminal FLAG Fusion Protein (protein-flag).

Applications: IP, Co-IP, pull-down, and ChIP assays

Packaging size: 500 μL / 1 mL / 5 mL

Features:

Low background—little to no nonspecific binding, and no preclearing
Highly reproducible—uniform beads ensure the most consistent results
Highly sensitive—Magbeads technology is the most cited method for sensitive applications, such as ChIP and IP,  of low abundance proteins
Fast and easy— no centrifugation or preclearing steps

Anti-DYKDDDDK (Flag) Affinity Gel - 20584ES
Anti-DYKDDDDK (Flag) Affinity Gel is prepared by conjugating high quality murine IgG2b monoclonal antibody with 4% highly cross-linked agarose gel. This product has a high Flag label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with Flag labels. Anti-DYKDDDDK (FLAG) Affinity Gel can bind to the Met modified N-flag fusion Protein (MET-flag-Protein), N-flag fusion protein (Flag-protein), C-terminal FLAG Fusion Protein (protein-flag).

Applications:
- Immunoprecipitation (IP)- Purification of fusion proteins with Flag labels.

Packaging size: 1 mL / 5 mL / 25 mL
* Note: In order to facilitate customers to use 1×Flag polypeptide elution, this product package is accompanied by 1×Flag polypeptide (Cat#20572ES). Based on the different specifications of 1×Flag polypeptide (Cat#20572ES), 1 mL gel package is combined with 5 mg peptide, and other gel specifications are combined with 1 mL: 1 mg ratio of 5 mg specification 1×Flag polypeptide (Cat#20572ES).

Features:

High Specificity—The selection of high quality murine IgG2b monoclonal antibody can specifically adsorb the Flag  protein sequence
high yield and high purity—Obtain high purity Flag label protein
High binding capacity—Capacity ≥1.1mg protein /mL gel
Many types of protein binding—Met-flag-Protein, N-flag fusion protein, C-terminal FLAG Fusion Protein
Widely used—Can be used for protein purification or IP

6X His Tag Peptide - 20576ES
6×His Tag Peptide is a polypeptide composed of 6 histidine residues with a high selective affinity for nickel ions in the side chain. It is used to competitively bind anti-6×His antibodies in immunoassay assay. The recommended working concentration of 6×His Tag peptides for elution of 6×His-Tag fusion proteins is 100-400 μg/mL. 

Packaging size: 5mg / 25mg

Features:                                                                             Applications:

High stability
High purity

Purify 6×His fusion proteins
Competitively bind Anti-6×His antibodies in immunoassay assay

3X Flag-tag Peptide - 20571ES
Flag polypeptide is a hydrophilic polypeptide encoding 8 amino acids (DYKDDDDK). It can be used to detect specific anti-Flag monoclonal antibodies. In order to improve the sensitivity of detection, 3×Flag repeated polypeptide (3× flag-tag Peptide) has been developed. This 3×Flag repeated polypeptide contains 23 amino acids and can detect fusion proteins up to 10 fmol. 3×Flag repeated peptides have been widely used to purify Flag fusion proteins and competitively bind Anti-Flag monoclonal antibodies. The recommended working concentration of 3×Flag repeated polypeptide is 100-400 μg/mL.

Packaging size: 4mg / 25mg

Features:                                                                             Applications:

High stability
High purity

Purify Flag fusion proteins
Competitively bind Anti-Flag monoclonal antibodies