Dextran Sulfate Sodium Salt (DSS) MW: 36000~50000 - 60316ES
Dextran sulfate sodium salt (DSS) is a polyanionic derivative of dextran produced by the esterification of Dextran with chlorosulphonic acid. The sulfur content is approximately 17% which corresponds to an average of 1.9 sulfate groups per glucosyl residue of the dextran molecule. DSS has several characteristics: 1) polyanionic complex, soluble in water, forming a colorless aqueous solution; 2) high purity and good stability; 3) can be degraded naturally. Inflammatory bowel disease (IBD) is a chronic, relapsing gastrointestinal infection that increases the risk of intestinal tumors, mainly including UC and Crohn disease (CD). In 1985, after the hamster ulcerative colitis model was first prepared by using DSS, a large number of studies have proved that the DSS colitis model is similar to human ulcerative colitis. The histological features, clinical manifestations, disease site, and cytokine proliferation of the DSS colitis model are very similar to human ulcerative colitis (UC). The modeling conditions and operation methods of this model are simple, the expenditure is very cheap, the repeatability is good, and it is easy to master and popularize. The DSS concentration and dosing time can be adjusted according to the experimental purpose to establish acute, chronic, and acute-chronic alternation models.
Packaging size: 25 g / 100 g / 500 g / 1 kg
Features:
- The protocols are easy to be implemented
- The DSS UC model closely resembles human UC symptoms with high repeatability
- Various characteristic symptoms can be induced by controlling the administrated DSS dose, which was unique for the DSS UC model
- The DSS UC model can be generated with a variety of widely used model animals, such as mice, rats, zebrafish, pigs, fruit flies, etc
- The IBD-induced colitis-associated cancer (CAC) model can be created with the combined use of azoxymethane (AOM)
Selected citations:[1] Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464.IF=16.744
[2] Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota[J].Theranostics.2021; 11(17): 8570-8586 IF=11.556
[3] Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
[4] Orally administrated nucleotide-delivery particles from microfluidics for inflammatory bowel disease treatment[J].Applied Materials Today.2021 Dec;25:101231 IF=10.041
[5] Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
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D-Luciferin, Sodium Salt D - 40901ES
D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging technology. The mechanism of action is that luciferin (the substrate) is oxidized to emit light in response to ATP and luciferase (see figure below). It's produced when there's too much luciferin The light quantum number was positively correlated with the concentration of luciferase. Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice In vivo, fluorescein is then injected and changes in light intensity are detected using bioluminescence imaging (BLI) to monitor disease progression or drug efficacy in real time and so on. ATP's influence on the reaction system can also be used to indicate energy or vital signs according to changes in bioluminescence intensity.
D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are currently three product forms: D-luciferin (free acid), D-luciferin salts (sodium and potassium salts). The main difference lies in solubility: the former is water-soluble and the solubility of buffer system is weak, except soluble in weak bases such as low concentration NaOH and KOH solutions, soluble in methanol and DMSO; The latter is easily soluble in water or buffer, easy to use, solvent non-toxic, especially suitable for in vivo experiments. As a solution, there is no substantial difference between the three products in the vast majority of applications. Applications: - In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.- To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.- The localization and distribution of foreign cells in animals were detected.- The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.- Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo. Packaging size: 100 mg / 500 mg / 1 g
D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are currently three product forms: D-luciferin (free acid), D-luciferin salts (sodium and potassium salts). The main difference lies in solubility: the former is water-soluble and the solubility of buffer system is weak, except soluble in weak bases such as low concentration NaOH and KOH solutions, soluble in methanol and DMSO; The latter is easily soluble in water or buffer, easy to use, solvent non-toxic, especially suitable for in vivo experiments. As a solution, there is no substantial difference between the three products in the vast majority of applications. Applications: - In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.- To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.- The localization and distribution of foreign cells in animals were detected.- The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.- Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo. Packaging size: 100 mg / 500 mg / 1 g
Features:
- No radiation, almost harmless to living organisms
- Bioluminescence, no excitation light source
- So sensitive, you can detect it in a few hundred cells
- Good penetration, 3-4cm tissue depth can still be detected
- High signal-to-noise ratio, strong fluorescence signal and good anti-interference
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Selected citations:
[1] Circ3823 contributes to growth, metastasis and angiogenesis of colorectal cancer: involvement of miR-30c-5p/TCF7 axis. Mol Cancer. 2021;20(1):93. Published 2021 Jun 25. doi:10.1186/s12943-021-01372-0(IF:27.401)
[2] Dynamic Adjust of Non-Radiative and Radiative Attenuation of AIE Molecules Reinforces NIR-II Imaging Mediated Photothermal Therapy and Immunotherapy. Adv Sci (Weinh). 2022;9(8):e2104793. doi:10.1002/advs.202104793(IF:16.806)
[3] Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA<sub>A</sub> R-Dependent TRIM21 Modulation of Src Expression. Adv Sci (Weinh). 2021;8(18):e2102079. doi:10.1002/advs.202102079(IF:16.806)
[4] Hydrogen sulfide-linked persulfidation of ABI4 controls ABA responses through the transactivation of MAPKKK18 in Arabidopsis. Mol Plant. 2021;14(6):921-936. doi:10.1016/j.molp.2021.03.007(IF:13.164)
[5] Potential of peptide-engineered exosomes with overexpressed miR-92b-3p in anti-angiogenic therapy of ovarian cancer. Clin Transl Med. 2021;11(5):e425. doi:10.1002/ctm2.425(IF:11.492)
[6] Arabidopsis cryptochrome 1 controls photomorphogenesis through regulation of H2A.Z deposition. Plant Cell. 2021;33(6):1961-1979. doi:10.1093/plcell/koab091(IF:11.277)
D-Luciferin, Potassium Salt - 40902ES
D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging Technology. The mechanism of action is that luciferin (substrate) can be oxidized to emit light in the presence of ATP and luciferase. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice.
D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are three Product form: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in solubility: water solubility of the former and solubility of the buffer system. They are weakly soluble except in weak bases such as low concentrations of NaOH and KOH solutions. Soluble in methanol and DMSO; The latter can be easily dissolved in water or buffer, easy to use. Applications: - In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.- To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.- The localization and distribution of foreign cells in animals were detected.- The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.- Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo. Packaging size: 100 mg / 500 mg / 1 g
D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are three Product form: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in solubility: water solubility of the former and solubility of the buffer system. They are weakly soluble except in weak bases such as low concentrations of NaOH and KOH solutions. Soluble in methanol and DMSO; The latter can be easily dissolved in water or buffer, easy to use. Applications: - In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.- To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.- The localization and distribution of foreign cells in animals were detected.- The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.- Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo. Packaging size: 100 mg / 500 mg / 1 g
Features:
- No radiation, almost harmless to living organisms
- Bioluminescence, no excitation light source
- So sensitive, you can detect it in a few hundred cells
- Good penetration, 3-4cm tissue depth can still be detected
- High signal-to-noise ratio, strong fluorescence signal and good anti-interference
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Selected citations:
[1] Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. Mol Cancer. 2021;20(1):132. Published 2021 Oct 14. doi:10.1186/s12943-021-01435-2(IF:27.401)
[2] A Novel Allosteric Inhibitor of Phosphoglycerate Mutase 1 Suppresses Growth and Metastasis of Non-Small-Cell Lung Cancer [published correction appears in Cell Metab. 2021 Jan 5;33(1):223]. Cell Metab. 2019;30(6):1107-1119.e8. doi:10.1016/j.cmet.2019.09.014(IF:22.415)
[3] A Heterojunction Structured WO<sub>2.9</sub>-WSe<sub>2</sub> Nanoradiosensitizer Increases Local Tumor Ablation and Checkpoint Blockade Immunotherapy upon Low Radiation Dose. ACS Nano. 2020;14(5):5400-5416. doi:10.1021/acsnano.9b08962(IF:14.588)
[4] A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11(1):1985. Published 2020 Apr 24. doi:10.1038/s41467-020-15927-0(IF:12.121)
[5] AUXIN RESPONSE FACTOR7 integrates gibberellin and auxin signaling via interactions between DELLA and AUX/IAA proteins to regulate cambial activity in poplar. Plant Cell. 2022;34(7):2688-2707. doi:10.1093/plcell/koac107(IF:11.277)