2× Hieff Canace™ AdvanceFast High-Fidelity PCR Master Mix (With Dye) - 10164ES
Ready-to-use 2× pre-mixed solution containing Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase, dNTPs, and an optimized buffer system, which contains pre-added electrophoresis indicators. The pre-mix contains pre-added electrophoresis indicator, PCR products can be directly electrophoresed, the amplification products are flat ends. 2× Hieff Canace™ AdvanceFast PCR Master Mix (With Dye) has the advantages of quick and easy, high sensitivity, high specificity, good stability, etc., the reaction system can be added with only the primers and templates. In addition, the product also contains a specific protective agent, so that the premix can still maintain stable activity after repeated freezing and thawing.
Figure: Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has high fidelity compared to other brands' products (A). The assay was done by examining the mutation frequency of PCR products of 6 GC-rich (about 80%) segments amplified from the genomes of different species. The PCR products amplified by Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase were cloned into vectors, and 100 selected single clones for each sequence were subjected to Sanger sequencing.
Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has high amplification speed (B), high GC amplification compatibility (C), and blood tolerance (D). Results from customers' PCR and sequencing show that Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has good performance in PCR yields and specificity (E-H).
Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has high amplification speed (B), high GC amplification compatibility (C), and blood tolerance (D). Results from customers' PCR and sequencing show that Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase has good performance in PCR yields and specificity (E-H).
Features:
- Gene cloning
- Amplification of complex templates DNA
- High-throughput library building.
Cas9 Nuclease - 14701ES
Cas9 Nuclease is derived from the wild-type Streptococcus pyogenes and is an RNA-guided endonuclease that specifically cleaves double-stranded DNA (it can also cleave single-stranded DNA or RNA in the presence of a DNA PAM). The Cas9 cleavage site is located within the target sequence, three base pairs away from the PAM (NGG) region. Cas9 Nuclease has undergone codon optimization and design with a nuclear localization signal (NLS), and is expressed through recombinant expression in Escherichia coli. It exhibits high editing efficiency and can be used for gene modification in cells (such as hematopoietic stem cells, T cells, etc.), as well as for molecular diagnostics and pathogen detection.
Applications:
- Genome editing based on CRISPR/Cas9 technology
- Gene modification of cells and gene therapy drugs (hematopoietic stem cells, T cells, etc.)
- In vitro screening of effective sgRNA
- In vitro cleavage of target DNA
Features:
- Optimized Core Positioning Signal: Enhanced NLS (Nuclear Localization Signal) for improved editing efficiency
- Consistent Editing Efficiency: Consistent high editing efficiency both in vitro and in vivo
- His Tag: His tag provides flexibility in fusion protein detection methods
- High Purity: Purity exceeding 95%
- High Concentration: Suitable for standard editing conditions and adaptable for optimizing editing conditions in challenging scenarios such as primary or embryonic cells, microinjection, or screening multiple gRNA sequences simultaneously.
Hifair™ Precision sgRNA Synthesis Kit - 11355ES
CRISPR/Cas9 is a technique in which RNA directs Cas nucleases to perform specific DNA modifications on targeted genes, resulting in an acquired immune defense mechanism evolved by bacteria and archaea in response to attacks by bacteriophages and exogenous plasmids. In modern biotechnology research, this system guides the nuclease Cas9 protein to cut double-stranded DNA at the target site paired with sgRNA by artificially optimized sgRNA (Single Guide RNA), causing DNA breaks. Due to the repair of DNA by non-homologous terminal repair mechanism or homologous recombination mechanism in organisms, gene shifting, mutation, replacement or deletion occur, resulting in loss of gene function.
The Hifair™ Precision sgRNA Synthesis Kit uses a T7 RNA polymerase to efficiently transcribe spCas9 sgRNA. This kit contains a specific sequence that is recognized by the Cas9 protein and acts as a scaffold, and part of this sequence can partially overlap with the user-designed specific sequence of interest. After annealing, complementary strands are formed, which are filled by DNA polymerase. The resulting dsDNA template for transcription is generated. Using the reagents provided and user-designed specific DNA sequences, this kit can obtain 20-100 μg of functional sgRNA in 4 hours in a single tube reaction.
The Hifair™ Precision sgRNA Synthesis Kit uses a T7 RNA polymerase to efficiently transcribe spCas9 sgRNA. This kit contains a specific sequence that is recognized by the Cas9 protein and acts as a scaffold, and part of this sequence can partially overlap with the user-designed specific sequence of interest. After annealing, complementary strands are formed, which are filled by DNA polymerase. The resulting dsDNA template for transcription is generated. Using the reagents provided and user-designed specific DNA sequences, this kit can obtain 20-100 μg of functional sgRNA in 4 hours in a single tube reaction.
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