DNA & RNA Extraction

MolPure® Cell/Tissue Total RNA Kit - 19221ES
Using MolPure® DNA removing / RNA binding column technology and new solution systems suitable for extracting high purity and quality total RNA from a variety of fresh or frozen animal tissues or cultured cells. The extraction process does not require toxic phenol, chloroform, β -mercaptoethanol, or precipitation of isopropanol and ethanol. Easy to operate, 15 min to complete the animal tissue or cells (10-30 mg animal tissue or (1-10) ×106 Animal cells) of total RNA extraction. The total RNA is of high purity and can be used in various molecular biology experiments including RT-PCR, qPCR, molecular cloning and RNase protection analysis.

Selected citations: 
[1] Lipid‐based Nanocarriers Enabled Oral Delivery of Oleanolic Acid Derivative DKS26 for Diabetes Management. Advanced Healthcare Materials.(2023)
[2] Overabundance of Veillonella parvula promotes intestinal inflammation by activating macrophages via LPS-TLR4 pathway. Cell Death Discov. 8, 251 (2022)

MolPure Magnetic Universal Viral DNA/RNA Kit - 18521ES
MolPureTM Magnetic Universal Viral DNA / RNA Kit is suitable for extracting viral nucleic acid from whole blood or cell-free body fluid samples (such as serum, plasma, cerebrospinal fluid, nasal / pharyngeal swab, alveolar lavage fluid, etc.). This method adopts magnetic bead purification technology, without toxic phenol chloroform extraction, safe, non-toxic and fast. The resulting products can be directly used for PCR, qPCR, second-generation sequencing and other experiments. With the automatic extraction instrument of the magnetic bead method, the high-throughput extraction of nucleic acid can be realized.

There are two packaging: bottled version and pre-packaged version (plates format).

Selected Citations:
[1] Vaginal Microbiome and Long and Short Outcomes of Cervical Balloon Catheter Induction of Labor: A Multicenter Prospective Cohort Study, 2024 DOI: https://doi.org/10.21203/rs.3.rs-4336060/v1

Ribonuclease A (RNase A) from bovine pancreas - 10407ES
Ribonuclease A (RNase A) is a single-stranded polypeptide containing 4 disulfide bonds with a molecular weight of about 13.7 kDa. RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. Specifically, the cleavage recognizes the phosphodiester bond formed by the 5'-ribose of a nucleotide and the phosphate group on the 3'-ribose of the adjacent pyrimidine nucleotide, so that the 2', 3' - Cyclic phosphates are hydrolyzed to the corresponding 3'-nucleoside phosphates (eg, pG-pG-pC-pA-pG is cleaved by RNase A to generate pG-pG-pCp and A-PG).RNase A is the most active in cleaving single-stranded RNA .Recommended working concentration is 1-100 μ G/mL, compatible with various reaction systems. Low salt concentration (0-100 mM NaCl) can be used to cut single-stranded RNA, double-stranded RNA, and RNA chains formed by RNA-DNA hybridization. However, at high salt concentration (≥ 0.3 M), RNase A only specifically cleaves single-stranded RNA.

RNase A is most commonly used to remove RNA during the preparation of plasmid DNA or genomic DNA. Whether or not DNase is active during the preparation process can easily affect the reaction. The traditional method of boiling in a water bath can be used to inactivate DNase activity. This product does not contain DNase and protease, and does not require heat treatment before use. In addition, this product can also be used in molecular biology experiments such as RNase protection analysis and RNA sequence analysis.

Packaging size: 100mg and 1g

Applications:
- Removal of RNA during genomic DNA preparation
- RNA enzyme protection analysis
- RNA sequence analysis

Proteinase K - 10401ES
Proteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl-terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29.3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common working concentration is 50-100 μg/mL, and the specific working concentration is according to whether the buffer contains SDS, urea, pH, temperature, and other factors.

Packaging size: 100mg and 1g

Applications:
- Genomic DNA extraction
- Digestion and removal of the enzyme

Selected Citations:
[1] Chimeric Antigen Receptor Designed to Prevent Ubiquitination and Downregulation Showed Durable Antitumor Efficacy. Immunity. 2020;53(2):456-470.e6. doi:10.1016/j.immuni.2020.07.011(IF:22.553)
[2] Autophagy-associated circular RNA hsa_circ_0007813 modulates human bladder cancer progression via hsa-miR-361-3p/IGF2R regulation. Cell Death Dis. 2021;12(8):778. Published 2021 Aug 7. doi:10.1038/s41419-021-04053-4(IF:8.469)