Mycoplasma Real-time qPCR Detection Kit - 40618ES
MycAway Mycoplasma Real-time qPCR Detection Kit is a product which can qualitative detect the mycoplasma contamination in the raw materials, cell bank, virus seeds, virual or cell harvesting solution and cells used in clinical treatment, etc. The kit uses the Taqman fluorescent probe (which included FAM and VIC) and the Multiple polymerase chain reaction (PCR) tools to detect the target and internal control separately. It covered over 100 species of the Mollicutes DNA, the specificity, detection limit and robustness of this kit are validated according to EP <2.6.7> which with high sensitivity, specificity, efficiency and safety. The detection limit is equals to and below 10 CFU/mL.
This product can be used in combination with MolPure® Magnetic Residual DNA Sample Preparation Kit (Cat#18461) which using the manual extracted method for the nucleic acid extraction. The nucleic acid in samples can also be automatically extracted by Auto-Pure 32A automatic nucleic acid extractor (Cat#80501) and MolPure® Mag32 Residual DNA Sample Preparation Kit FA(Cat#18462)(Please note, the kits which included Cat#18461 and Cat#40618 are full validated, please contact our technical support for detailed validation information). After the samples are pre-treated to remove the interference impurities and obtain purified nucleic acid, then a qPCR reaction perform by the Real Time PCR amplifier and the fluorescence signal of the probe will be collected and analyzed. Manual Packaging size: 25 T / 100 T
This product can be used in combination with MolPure® Magnetic Residual DNA Sample Preparation Kit (Cat#18461) which using the manual extracted method for the nucleic acid extraction. The nucleic acid in samples can also be automatically extracted by Auto-Pure 32A automatic nucleic acid extractor (Cat#80501) and MolPure® Mag32 Residual DNA Sample Preparation Kit FA(Cat#18462)(Please note, the kits which included Cat#18461 and Cat#40618 are full validated, please contact our technical support for detailed validation information). After the samples are pre-treated to remove the interference impurities and obtain purified nucleic acid, then a qPCR reaction perform by the Real Time PCR amplifier and the fluorescence signal of the probe will be collected and analyzed. Manual Packaging size: 25 T / 100 T
Features:
- Detection Limit: 10 CFU/mL
- Detection Method: qPCR method (Taqman fluorescent)
- Fluorescent Probe: FAM (Target channel)；VIC (Internal channel)
- Duration: 4 hours
- Covered mycoplasma: ≥ 100
- Validation: Validated according to EP <2.6.7>
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