Biopharma - In Vitro Transcription

T7 RNA Polymerase GMP-grade (50 U/μL) - 10624ES
This product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA.Note: G* is the first base of the RNA transcript.
This product is produced in accordance with GMP regulations and provided in liquid form.

Packaging size: 5000 U / 50000 U

Features:                                                                             Applications:

Synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template
Higher yield and easy to operate
Lower endotoxins
Tested for the absence of endonucleases, exonucleases, RNases

Radiolabeled RNA probe preparation
RNA generation for studies of RNA structure, processing and catalysis
Expression control via anti-sense RNA
mRNA, sgRNA synthesis

T7 RNA Polymerase GMP-grade (250 U/μL) - 10625ES
This product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA.Note: G* is the first base of the RNA transcript.
This product is produced in accordance with GMP regulations and provided in liquid form.

Packaging size: 10 KU / 100 KU

Features:                                                                             Applications:

Synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template
Higher yield and easy to operate
Lower endotoxins
Tested for the absence of endonucleases, exonucleases, RNases

Radiolabeled RNA probe preparation
RNA generation for studies of RNA structure, processing and catalysis
Expression control via anti-sense RNA
mRNA, sgRNA synthesis

T7 High Yield RNA Synthesis Kit - 10623ES
T7 High Yield RNA Synthesis Kit optimizes the transcription reaction system. The kit can synthesize the single-stranded RNA efficiently by using T7 RNA polymerase, the linear double-stranded DNA with the T7 promoter sequence as the template, NTPs as the substrate to control the DNA sequence downstream of the promoter. During transcription, modified nucleotides can be added to the substrate to prepare biotin or dye-labeled RNA.
This kit can synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template input. The RNA synthesized by transcription can be used for various downstream applications, such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation.

Packaging: 10 T / 50 T / 100 T

Features:

Up to 180 μg of RNA per reaction from 1 μg of the control template
Optimized reaction system for the IVT process
Decrease the dsRNA production
Higher RNA integrity and purity

10× Transcription Buffer 2 GMP-grade - 10670ES
This product is an optimized transcription buffer and is efficient for in vitro synthesis of RNA. This product is mainly composed of buffer salts, reducing agents, and magnesium ions required for enzyme proteins. This product is produced by GMP regulations and provided in liquid form.

Packaging: 1 mL / 10 mL / 25 mL/ 500 mL
 
10×Transcription Buffer GMP-grade (Mg2+ free) - 10669ESThis product is an optimized transcription buffer and efficient for in vitro synthesis of RNA. The formulation of the product: 400mM Tris-HCl, 20mM spermidine, 100mM DTT, pH 7.9 at 25℃. This product is produced in accordance with GMP regulations and provided in liquid form. 
Packaging: 1 mL / 10 mL / 100 mL

Recombinant Deoxyribonuclease I  (DNase I，RNase-free) GMP-grade (2 U/μL) - 10611ES
DNase I is an endonuclease that can digest single-stranded and double-stranded DNA to produce single deoxynucleotides or single-stranded or double-stranded oligodeoxynucleotides. It can hydrolyze the phosphodiester bond to produce monodeoxynucleotides and oligodeoxynucleotides containing 5'-phosphate groups and 3'-OH groups. The average digestion product is the smallest polytetranucleotide. DNase I can catalyze many forms of DNA, such as single-stranded DNA, double-stranded DNA, and even chromatin (its cutting rate is affected by histones). The optimum pH range is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions, such as Co2+, Mn2+, Zn2+, etc. 5 mM Ca2+ can protect the enzyme from being hydrolyzed. In the presence of Mg2+, the enzyme can recognize and cut any site on any strand of DNA randomly; and in the presence of Mn2+, it can recognize two strands of DNA at the same time and cut at almost the same site to form blunt ends, Or sticky ends with 1-2 nucleotides protruding. DNase I is widely used in the preparation of DNA-free RNA; remove the template DNA after in vitro transcription; prepare DNA-free RNA before RT-PCR and RT-qPCR reactions; combine with DNA polymerase I to perform DNA labeling through nick translocation; DNA fragmentation library construction.
This product is produced in accordance with GMP process requirements, and the product is provided in liquid form.

Packaging size: 500 U / 2000 U / 10000 U

Features:                                                                             Applications:

DNA specific Endonuclease
Degrades double-stranded and single-stranded DNA
Products are short oligos with 5'-phosphate and 3'-OH

Removal of contaminating genomic DNA from RNA samples
Degradation of DNA templates in transcription reactions

Selected citations:
[1] METTL14 aggravates podocyte injury and glomerulopathy progression through N<sup>6</sup>-methyladenosine-dependent downregulating of Sirt1. Cell Death Dis. 2021;12(10):881. Published 2021 Sep 27. doi:10.1038/s41419-021-04156-y(IF:8.469)
[2] Biomimetic periosteum-bone substitute composed of preosteoblast-derived matrix and hydrogel for large segmental bone defect repair. Acta Biomater. 2020;113:317-327. doi:10.1016/j.actbio.2020.06.030(IF:7.242)

Murine RNase inhibitor GMP-grade (40 U/μL) - 10621ES
RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase. This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.). This product is produced in accordance with GMP process requirements and is provided in liquid form.

Packaging size: 10 U / 20 U / 100 KU / 1 MU

Features:                                                                             Applications:

Improved resistance to oxidation, compared to human/porcine RNase inhibitor
Ideal for reactions where low DTT concentrations are required (e.g., Real-time PCR)
Isolated from a recombinant source
Tested for the absence of DNases and RNases

RT-PCR
cDNA synthesis
In vitro transcription/translation
Enzymatic RNA labeling reaction
Other applications where the integrity of RNA is important

Selected citations:
[1] Natural polyphenol assisted delivery of single-strand oligonucleotides by cationic polymers. Gene Ther. 2020;27(7-8):383-391. doi:10.1038/s41434-020-0151-y(IF:4.128)

Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL) - 10672ES
This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reactions.This product is produced by GMP process requirements, and the product is provided in liquid form

Packaging: 1 U / 10 U / 100 U

Pyrophosphatase, Inorganic GMP-grade (1 U/μL) - 10620ES
This product is an inorganic pyrophosphatase derived from recombinant expression in Escherichia coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reactions. This product is produced in accordance with GMP process requirements, and the product is provided in liquid form.

Packaging size: 10 U / 100 U / 1000 U / 40 KU

Features:                                                                             Applications:

Isolated from a recombinant source
Tested for the absence of endonucleases, exonucleases, RNases
Increasing RNA yield in transcription reaction; enhancing DNA replication

DNA sequencing reaction
PCR and single-base extension reaction
In vitro transcription processes

NTP Set Sodium Solution (ATP, CTP, UTP, GTP, 100 mM each) - 10133ES
The NTP Set Solution is a convenient set of 100 mM aqueous solutions of each of ATP, CTP, UTP, GTP, supplied in separate vials. They can be used in a variety of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc, additionally, used as a reaction substrate or coenzyme for a variety of enzymes.
This product is a clear colorless solution prepared from trisodium salts of ATP, UTP, GTP and CTP with a purity of ≥99%, pH = 7.0±0.1 (25°C), concentration of 100 mM, and is DNase-free and RNase-free.

Packaging size: 1 set (4 vials)

Features:

Validated, product-specific process and analytical methods
Product-specific stability
Documentation follows applicable GMP guidelines
AOF production process and raw materials (TSE & BSE) 
Nitrosamine statement
Regulatory support documents available
Large-scale production
Nucleotide in the multiple salt form（Na+, Tris etc) always available to meet different downstream application needs