Protein interaction profile sequencing (PIP-seq) is a genome-wide method that uses structure-specific ribonucleases (RNases) to identify regions of double-stranded and single-stranded RNA as well as regions that are protected from these RNases, which represent sites of RBP binding.
The ADCC (Antibody-dependent cellular cytotoxicity (ADCC)) reporter gene assay is a bioluminescent reporter gene assay used to quantify the biological activity of antibodies. The assay uses effector cells, Jurkat cells, which stably express the human FcγRIIIa receptor and the NFAT response element (NFAT-RE) that drives the expression of firefly luciferase. When the antibody binds to the antigen on the target cell and the FcγRIIIa receptor on the ADCC bioassay effector cell, it leads to the activation of FcγRIIIa and the subsequent expression of luciferase in the ADCC bioassay effector cell.
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In the process of antibody drug development, it is necessary to detect the obtained antibodies and evaluate the efficacy of antibodies or target cells.
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Appropriate preservation and storage of stool samples is crucial to maintain DNA integrity and microbial community composition for downstream applications and analysis, including NGS and Microbiome characterization.
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CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats Cas9) is a system composed of clustered regularly interspaced short palindromic repeats and Cas9 protein, which is the immune system of bacteria to resist foreign viral infection. Modern biology has widely used the CRISPR/Cas9 system in the field of gene editing . It is the most important gene modification technology after zinc-finger nuclease (ZFN) and transcription activator- like effector nuclease ( TALENs). It can be widely used in gene knock-in, gene knock-out, gene activation, gene silencing, epigenetic modification and 3D gene structure change.
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Collagenase is a type of endopeptidase that can specifically recognize the Pro-X-Gly-Pro sequence (this sequence appears frequently in collagen and is rarely found in other proteins) and cleave the peptide bond between the neutral amino acid (X) and glycine (Gly) in this sequence. It can also hydrolyze collagen in connective tissue.
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Cytokines are a class of small molecule proteins with extensive biological activity that are synthesized and secreted by immune cells after stimulation.
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Apoptosis is a type of programmed cell death, which is strictly controlled by genes and is essential for controlling cell growth, development and renewal. It can include a series of characteristic changes, such as morphological changes such as cell shrinkage, nuclear condensation, nuclear fragmentation, and apoptotic body formation, as well as biochemical changes such as characteristic DNA fragmentation, new gene expression, and synthesis of certain biomacromolecules.
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The choice between one-step and two-step RT-qPCR depends on your experimental goals: one-step is faster and simpler for high-throughput applications with a limited number of targets, while two-step offers more flexibility and sensitivity for analyzing multiple targets or optimizing cDNA synthesis.
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Enzyme-linked immunosorbent assay ( ELISA ) is the most widely used technology in enzyme immunoassay technology . The invention of ELISA Kit has helped scientific researchers save the tedious experimental process and improved scientific research efficiency. Choosing the right kit and combining good experimental skills will make your experimental efficiency twice as good with half the effort.
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Take your protein research to the next level with Yeasen’s high-quality reagents and kits, designed for Western blot, ELISA, protein purification, and electrophoresis.
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