Apoptosis & Proliferation
Reactive Oxygen Species Assay Kit - 50101ES
Reactive Oxygen Species Assay Kit uses the cell permeant reagent 2’,7’–dichlorofluorescin diacetate (DCFDA) to quantitatively assess reactive oxygen species in live cell samples. The lipophilic non-fluorescent DCFDA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later by ROS to form DCF. DCF is highly fluorescent and is detected by fluorescence spectroscopy or Flow cytometry with excitation/emission at 480 nm/525 nm. Rosup, a compound mixture, is a ROS inducer and can be used as a positive control.
Packaging size: 1000 T
Selected citations:
[1] Conscription of Immune Cells by Light-Activatable Silencing NK-Derived Exosome (LASNEO) for Synergetic Tumor Eradication. Adv Sci (Weinh). 2022 Aug;9(22): e2201135. doi: 10.1002/advs.202201135. Epub 2022 Jun 4. IF: 16.806
[2] Microalgae-based oral microcarriers for gut microbiota homeostasis and intestinal protection in cancer radiotherapy. Nat Commun. 2022 Mar 17;13(1):1413. doi: 10.1038/s41467-022-28744-4. PMID: 35301299. IF: 14.919
[3] Biocompatible reduced graphene oxide stimulated BMSCs induce acceleration of bone remodeling and orthodontic tooth movement through promotion on osteoclastogenesis and angiogenesis. Bioact Mater. 2022 Feb 6; 15:409-425. doi: 10.1016/j.bioactmat.2022.01.021. PMID: 35386350; PMCID: PMC8958387. IF: 14.593
[4] Space-Selective Chemodynamic Therapy of CuFe5O8 Nanocubes for Implant-Related Infections. ACS Nano. 2020 Oct 27;14(10):13391-13405. doi: 10.1021/acsnano.0c05255. Epub 2020 Sep 22. PMID: 32931252. IF: 14.588
[5] Red Phosphorus Decorated TiO2 Nanorod Mediated Photodynamic and Photothermal Therapy for Renal Cell Carcinoma. Small. 2021 Jul;17(30): e2101837. doi: 10.1002/smll.202101837. Epub 2021 Jun 19. PMID: 34145768. IF：13.281
.JPG@webp)
Alamar Blue - 40202ES
Alamar Blue is an oxidative reducing indicator that can generate changes and fluorescent signals according to metabolic activity. This is a safe and non -toxic dye that can be used for quantitative analysis of cell activity and cell proliferation as well as cytokine biological determination and in vitro cytotoxicity. It can effectively determine the natural metabolic activity of animals, fungi and bacterial cells. Alamar Blue is purple -blue without fluorescence in the oxidation state. In the restore state, it transforms into a pink or red fluorescent restoration product, reflecting the consumption of oxygen molecules studied by the cells or microorganisms studied, so it is often used as oxidation. Restore the indicator to show metabolic activities observed cells and bacteria. This determination is the ability to convert reagents into fluorescence and color -color indicator based on metabolic activity. During the process of cell proliferation, the ratio of NADPH/NADP, FADH/FAD, FMNH/FMN, and NADH/NAD increased in the cells. The dyes intake in the cells are restored by these metabolic intermediates and cytochromes and release them to the outer cells and dissolved in the medium, so that the medium will change from a fluorescent blue blue to fluorescent pink. Damage and active cells have low natural metabolic activity, so the corresponding signals are lower. It can be detected with ordinary optical light meter or fluorescent light meter. It stimulates the wavelength between 530-560 nm and the transmitting light wavelength is 590 nm. Inhalery and fluorescent strength are directly proportional to the number of active cells.
This product can be widely used for cell proliferation metabolism, in vitro measurement of cytotoxic effects of drugs, and rapid detection and identification of pathogenic microorganisms. Compared with the analysis methods such as Taipanlan, TTC, MTT, MTS, etc., Alamar Blue has more advantages. Alamar Blue uses a single reagent to continuously and quickly detect the hyperplasia of cells. Because Alamar Blue is non -toxic and harmless to the cells, and does not affect the activity of antibody synthesis and secretion of cells, it can be continuously observed and further experimental observation of the hyperplasia of the same batch of cells. Therefore The characteristics of metabolism. Packaging size: 1000T (10mL) / 10000T (100mL)
This product can be widely used for cell proliferation metabolism, in vitro measurement of cytotoxic effects of drugs, and rapid detection and identification of pathogenic microorganisms. Compared with the analysis methods such as Taipanlan, TTC, MTT, MTS, etc., Alamar Blue has more advantages. Alamar Blue uses a single reagent to continuously and quickly detect the hyperplasia of cells. Because Alamar Blue is non -toxic and harmless to the cells, and does not affect the activity of antibody synthesis and secretion of cells, it can be continuously observed and further experimental observation of the hyperplasia of the same batch of cells. Therefore The characteristics of metabolism. Packaging size: 1000T (10mL) / 10000T (100mL)
Cell Counting Kit (CCK-8) - 40203ES
Cell Counting Kit-8 (CCK-8) is a fast and highly sensitive detection kit based on the WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt) reagent. WST-8 is an upgraded reagent of MTT and can be reduced to a highly water-soluble orange-yellow formazan by dehydrogenases in the mitochondria. The amount of the formazan generated is proportional to the number of living cells. The OD value of formazan at 450nm detected by a microplate reader can indirectly indicate the number of viable cells. This kit is widely used for drug screening, cell proliferation and cytotoxicity assays, tumor drug sensitivity testing, and activity detection of biological factors.
Kindly refer the Advantages of CCK-8 method compare with other cell proliferation/toxicity detection methods on the right table. Phenol red and serum do not interfere with CCK-8 detection. As the cytotoxicity of CCK-8 reagent is very low, the optimal measurement time can be determined by repeatedly reading with a microplate reader at different times after adding CCK-8 reagent.
Packaging: 100T / 500T / 1000T
Selected citations:
[1] In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. Cell. 2021;184(7):1865-1883.e20. doi:10.1016/j.cell.2021.02.008(IF:41.584)
[2] GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation. Cell Discov. 2022;8(1):77. Published 2022 Aug 9. doi:10.1038/s41421-022-00423-0(IF:38.079)
[3] Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293. doi:10.1038/s41587-019-0249-1(IF:31.864)
[4] Ferroptosis heterogeneity in triple-negative breast cancer reveals an innovative immunotherapy combination strategy [published online ahead of print, 2022 Oct 11]. Cell Metab. 2022;S1550-4131(22)00411-9. doi:10.1016/j.cmet.2022.09.021(IF:31.373)
[5] GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma. Mol Cancer. 2021;20(1):80. Published 2021 May 29. doi:10.1186/s12943-021-01374-y(IF:27.401)
.JPG@webp)
TUNEL Apoptosis Detection Kit (FITC) - 40306ES
When cells undergo apoptosis, they activate endonuclease enzymes that cut genomic DNA between nucleosomes. DNA ladder of 180-200 bp can be detected by electrophoresis after extraction of DNA during apoptosis.
TUNEL (TdT mediated dUTP Nick End Labeling) Cell apoptosis Assay Kit (FITC) can be used to detect the nuclear DNA breakage in tissue cells during late apoptotic stage. The principle is that FITC-12-dUTP is inserted into the 3'-hydroxyl (3'-OH) terminal of genomic DNA exposed during DNA break under the action of Terminal Deoxynucleotidyl Transferase (TdT). This can be detected by fluorescence microscopy or flow cytometry.
This kit optimizes the labeling reaction by using FITC-12-dUTP and unlabeled dNTP at the optimal ratio for nucleotide incorporation at the 3' -OH terminal, resulting in a longer "labeling tail" at the end of the same broken DNA fragment. The “labeling tail” reduces the steric hindrance of labeled groups on adjacent dNTP, increases the number of fluorescent-groups on each broken fragment, and reduces the aggregation and quenching that may be caused by adjacent fluorescent-groups, thus improving detection sensitivity and reducing non-specific reactions.
This kit has a wide range of applications. It can be used to detect apoptosis in frozen or paraffin sections, as well as in culture adherent cells or suspension cells situation. Packaging size: 20T/ 50T / 100T Selected citations: [1] A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov. 2017;3:17044. Published 2017 Dec 5. doi:10.1038/celldisc.2017.44(IF:10.849) [2] Passion fruit-like exosome-PMA/Au-BSA@Ce6 nanovehicles for real-time fluorescence imaging and enhanced targeted photodynamic therapy with deep penetration and superior retention behavior in tumor. Biomaterials. 2020;230:119606. doi:10.1016/j.biomaterials.2019.119606(IF:10.273) [3] Therapeutic silencing miR-146b-5p improves cardiac remodeling in a porcine model of myocardial infarction by modulating the wound reparative phenotype. Protein Cell. 2021;12(3):194-212. doi:10.1007/s13238-020-00750-6(IF:10.164) [4] Niacin ameliorates ulcerative colitis via prostaglandin D<sub>2</sub>-mediated D prostanoid receptor 1 activation [published correction appears in EMBO Mol Med. 2020 Dec 7;12(12):e13487]. EMBO Mol Med. 2017;9(5):571-588. doi:10.15252/emmm.201606987(IF:9.249) [5] Novel Multifunctional Silver Nanocomposite Serves as a Resistance-Reversal Agent to Synergistically Combat Carbapenem-Resistant Acinetobacter baumannii. ACS Appl Mater Interfaces. 2021;13(26):30434-30457. doi:10.1021/acsami.1c10309(IF:9.229)
TUNEL (TdT mediated dUTP Nick End Labeling) Cell apoptosis Assay Kit (FITC) can be used to detect the nuclear DNA breakage in tissue cells during late apoptotic stage. The principle is that FITC-12-dUTP is inserted into the 3'-hydroxyl (3'-OH) terminal of genomic DNA exposed during DNA break under the action of Terminal Deoxynucleotidyl Transferase (TdT). This can be detected by fluorescence microscopy or flow cytometry.
This kit optimizes the labeling reaction by using FITC-12-dUTP and unlabeled dNTP at the optimal ratio for nucleotide incorporation at the 3' -OH terminal, resulting in a longer "labeling tail" at the end of the same broken DNA fragment. The “labeling tail” reduces the steric hindrance of labeled groups on adjacent dNTP, increases the number of fluorescent-groups on each broken fragment, and reduces the aggregation and quenching that may be caused by adjacent fluorescent-groups, thus improving detection sensitivity and reducing non-specific reactions.
This kit has a wide range of applications. It can be used to detect apoptosis in frozen or paraffin sections, as well as in culture adherent cells or suspension cells situation. Packaging size: 20T/ 50T / 100T Selected citations: [1] A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov. 2017;3:17044. Published 2017 Dec 5. doi:10.1038/celldisc.2017.44(IF:10.849) [2] Passion fruit-like exosome-PMA/Au-BSA@Ce6 nanovehicles for real-time fluorescence imaging and enhanced targeted photodynamic therapy with deep penetration and superior retention behavior in tumor. Biomaterials. 2020;230:119606. doi:10.1016/j.biomaterials.2019.119606(IF:10.273) [3] Therapeutic silencing miR-146b-5p improves cardiac remodeling in a porcine model of myocardial infarction by modulating the wound reparative phenotype. Protein Cell. 2021;12(3):194-212. doi:10.1007/s13238-020-00750-6(IF:10.164) [4] Niacin ameliorates ulcerative colitis via prostaglandin D<sub>2</sub>-mediated D prostanoid receptor 1 activation [published correction appears in EMBO Mol Med. 2020 Dec 7;12(12):e13487]. EMBO Mol Med. 2017;9(5):571-588. doi:10.15252/emmm.201606987(IF:9.249) [5] Novel Multifunctional Silver Nanocomposite Serves as a Resistance-Reversal Agent to Synergistically Combat Carbapenem-Resistant Acinetobacter baumannii. ACS Appl Mater Interfaces. 2021;13(26):30434-30457. doi:10.1021/acsami.1c10309(IF:9.229)
Features: Applications:
- The reagents in this kit are provided in liquid form for easy use
- The kit can be used for a variety of purposes: flow cytometry, fluorescence microscopy for detection
- Under the fluorescence microscope, the color distinction is obvious
- This kit can be used for flow cytometry and fluorescence microscopy
- This kit can detect the early apoptosis of cells
- This kit can distinguish between late apoptotic and necrotic cells
.JPG@webp)
.JPG@webp)
TUNEL Apoptosis Detection Kit (Alexa Fluor 640) - 40308ES
When cells undergo apoptosis, they activate endonuclease enzymes that cut the genomic DNA between nucleosomes. After DNA was extracted during apoptosis, a 180-200 bp DNA ladder could be found. TUNEL (TdT mediated dUTP Nick End Labeling) Apoptosis detection kit (Alexa Fluor 640) can be used to detect nuclear DNA fragmentation in the late apoptotic process of tissue cells. The principle is that under the action of Terminal Deoxynucleotidyl Transferase (TdT), Alexa Fluor 640-12-DUTP is incorporated into the 3´ -hydroxyl (3´-OH) Terminal exposed during genomic DNA break. Thus, it can be detected by fluorescence microscopy or flow cytometry. Alexa Fluor 640 is a red fluorescent dye with high stability and strong brightness.
This kit has a wide range of applications and can be used to detect apoptosis in frozen or paraffin sections, as well as in cultured adherent or suspended cells.
Packaging size: 20T/ 50T / 100T
.JPG@webp)
TUNEL Apoptosis Detection Kit (Alexa Fluor 488) - 40307ES
When cells undergo apoptosis, they activate endonuclease enzymes that cut the genomic DNA between nucleosomes. DNA ladder of 180-200bp was detected by electrophoresis after DNA extraction during apoptosis. TUNEL (TdT mediated dUTP Nick End Labeling) Apoptosis detection kit (Alexa Fluor 488) can be used to detect nuclear DNA fragmentation in the late apoptotic process of tissue cells. The principle is that under the action of Terminal Deoxynucleotidyl Transferase (TdT), Alexa Fluor 488-12-DUTP is incorporated into the 3´ -hydroxyl (3´-OH) Terminal exposed during genomic DNA break. Thus, it can be detected by fluorescence microscopy or flow cytometry.
Alexa Fluor 488 dye is more stable and has a stronger signal, resulting in brighter markers and greater resistance to quench.This kit has a wide range of applications and can be used to detect apoptosis in frozen or paraffin sections, as well as in cultured adherent or suspended cells.
Packaging size: 20T/ 50T / 100T
.JPG@webp)
Annexin V-FITC/PI Apoptosis Detection Kit - 40302ES
Annexin V-FITC/PI Apoptosis Detection Kit uses FITC-labeled Annexin V as a probe to detect early apoptosis of cells. The detection principle is that in normal living cells, phosphotidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS reverses from the inner side to the surface of the cell membrane and is exposed to the extracellular environment. Annexin V is a Ca2+ -dependent phospholipid binding protein with a molecular weight of 35-36 kDa. Annexin V is a Ca2+ -dependent phospholipid binding protein with a high affinity for PS and binds to the membranes of early-apoptotic cells via externally exposed phosphatidylserine.
In addition, Propidium Iodide (PI) is provided in this kit to distinguish between surviving early cells and necrotic or late apoptotic cells. PI is a kind of nucleic acid dye, which can not penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells and make the nucleus red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-). The apoptotic cells and necrotic cells were double positive by FITC and PI binding staining (Annexin V+/PI+). This kit can be used for flow cytometry and fluorescence microscopy. Packaging size: 20T/ 50T / 100T Selected citations: [1] Human pluripotent stem-cell-derived islets ameliorate diabetes in non-human primates. Nat Med. 2022;28(2):272-282. doi:10.1038/s41591-021-01645-7(IF:53.440) [2] SIDT1-dependent absorption in the stomach mediates host uptake of dietary and orally administered microRNAs. Cell Res. 2021;31(3):247-258. doi:10.1038/s41422-020-0389-3(IF:25.617) [3] Dynamic Adjust of Non-Radiative and Radiative Attenuation of AIE Molecules Reinforces NIR-II Imaging Mediated Photothermal Therapy and Immunotherapy. Adv Sci (Weinh). 2022;9(8):e2104793. doi:10.1002/advs.202104793(IF:16.806) [4] Conscription of Immune Cells by Light-Activatable Silencing NK-Derived Exosome (LASNEO) for Synergetic Tumor Eradication [published online ahead of print, 2022 Jun 4]. Adv Sci (Weinh). 2022;e2201135. doi:10.1002/advs.202201135(IF:16.806) [5] Oxygen-Delivering Polyfluorocarbon Nanovehicles Improve Tumor Oxygenation and Potentiate Photodynamic-Mediated Antitumor Immunity. ACS Nano. 2021;15(3):5405-5419. doi:10.1021/acsnano.1c00033(IF:15.881)
In addition, Propidium Iodide (PI) is provided in this kit to distinguish between surviving early cells and necrotic or late apoptotic cells. PI is a kind of nucleic acid dye, which can not penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells and make the nucleus red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-). The apoptotic cells and necrotic cells were double positive by FITC and PI binding staining (Annexin V+/PI+). This kit can be used for flow cytometry and fluorescence microscopy. Packaging size: 20T/ 50T / 100T Selected citations: [1] Human pluripotent stem-cell-derived islets ameliorate diabetes in non-human primates. Nat Med. 2022;28(2):272-282. doi:10.1038/s41591-021-01645-7(IF:53.440) [2] SIDT1-dependent absorption in the stomach mediates host uptake of dietary and orally administered microRNAs. Cell Res. 2021;31(3):247-258. doi:10.1038/s41422-020-0389-3(IF:25.617) [3] Dynamic Adjust of Non-Radiative and Radiative Attenuation of AIE Molecules Reinforces NIR-II Imaging Mediated Photothermal Therapy and Immunotherapy. Adv Sci (Weinh). 2022;9(8):e2104793. doi:10.1002/advs.202104793(IF:16.806) [4] Conscription of Immune Cells by Light-Activatable Silencing NK-Derived Exosome (LASNEO) for Synergetic Tumor Eradication [published online ahead of print, 2022 Jun 4]. Adv Sci (Weinh). 2022;e2201135. doi:10.1002/advs.202201135(IF:16.806) [5] Oxygen-Delivering Polyfluorocarbon Nanovehicles Improve Tumor Oxygenation and Potentiate Photodynamic-Mediated Antitumor Immunity. ACS Nano. 2021;15(3):5405-5419. doi:10.1021/acsnano.1c00033(IF:15.881)
Features: Applications:
- The reagents in this kit are provided in liquid form for easy use
- The kit can be used for a variety of purposes: flow cytometry, fluorescence microscopy for detection
- Under the fluorescence microscope, the color distinction is obvious
- This kit can be used for flow cytometry and fluorescence microscopy
- This kit can detect the early apoptosis of cells
- This kit can distinguish between late apoptotic and necrotic cells
.JPG@webp)
.JPG@webp)
Annexin V-EGFP/PI Apoptosis Detection Kit - 40303ES
Annexin V-EGFP/PI cell apoptosis detection kit uses EGFP-labeled Annexin V as a probe to detect the occurrence of early cell apoptosis.The detection principle is as follows: in normal living cells, phosphotidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS flips from the inner side of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin-V is a Ca2+ -dependent phospholipid-binding protein with a molecular weight of 35 to 36 kDa that binds to PS with high affinity. The phosphatidyl serine can bind to the membrane of early apoptotic cells through the exposed phosphatidyl serine on the outside of the cell.
In addition, Propidium Iodide (PI) is provided to distinguish surviving early cells from necrotic or late apoptotic cells. PI is a nucleic acid dye, which can not pass through the intact cell membrane of normal cells or early apoptotic cells but can pass through the cell membrane of late apoptotic and necrotic cells and make the nucleus red. Thus, when Annexin V was combined with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-). The late apoptotic and necrotic cells were double positive for both EGFP and PI (Annexin V+/PI+). This kit is suitable for flow cytometry and fluorescence microscopy.
Packaging size: 20T/ 50T / 100T
.JPG@webp)
.JPG@webp)
Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit - 40304ES
Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit is an Annexin Fluor 647 labeled with Annexin V as a probe to detect the occurrence of early apoptosis, which can be detected by flow cytometry or other fluorescence detection equipment. The detection principle is that in normal living cells, phosphotidylserine (PS) is located on the inner side of the cell membrane, but in early apoptotic cells, PS reverses from the inner side to the surface of the cell membrane and is exposed to the extracellular environment. Annexin V is a Ca2+ -dependent phospholipid binding protein with a molecular weight of 35-36 kD that binds PS with high affinity. Phosphatidylserine can bind to the membrane of early-apoptotic cells by exposing it laterally.
In addition, Propidium Iodide (PI) is provided in this kit to distinguish between surviving early cells and necrotic or late apoptotic cells. PI is a kind of nucleic acid dye, which can not penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells and make the cell nucleus red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-). The apoptotic and necrotic cells were double positive by Alexa Fluor 647 and PI binding staining (Annexin V+/PI+).This kit requires flow cytometry. Packaging size: 20T/ 50T / 100T Selected citations: [1] PD-L1 lncRNA splice isoform promotes lung adenocarcinoma progression via enhancing c-Myc activity. Genome Biol. 2021;22(1):104. Published 2021 Apr 13. doi:10.1186/s13059-021-02331-0(IF:13.583) [2] Tumor-derived miR-378a-3p-containing extracellular vesicles promote osteolysis by activating the Dyrk1a/Nfatc1/Angptl2 axis for bone metastasis. Cancer Lett. 2022;526:76-90. doi:10.1016/j.canlet.2021.11.017(IF:8.679) [3] Macroporous organosilicon nanocomposites co-deliver Bcl2-converting peptide and chemotherapeutic agent for synergistic treatment against multidrug resistant cancer. Cancer Lett. 2020;469:340-354. doi:10.1016/j.canlet.2019.10.018(IF:6.508) [4] Nobiletin mitigates hepatocytes death, liver inflammation, and fibrosis in a murine model of NASH through modulating hepatic oxidative stress and mitochondrial dysfunction. J Nutr Biochem. 2022;100:108888. doi:10.1016/j.jnutbio.2021.108888(IF:6.048) [5] eIF4A1 Inhibitor Suppresses Hyperactive mTOR-Associated Tumors by Inducing Necroptosis and G2/M Arrest. Int J Mol Sci. 2022;23(13):6932. Published 2022 Jun 22. doi:10.3390/ijms23136932(IF:5.924)
In addition, Propidium Iodide (PI) is provided in this kit to distinguish between surviving early cells and necrotic or late apoptotic cells. PI is a kind of nucleic acid dye, which can not penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells and make the cell nucleus red. Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-). The apoptotic and necrotic cells were double positive by Alexa Fluor 647 and PI binding staining (Annexin V+/PI+).This kit requires flow cytometry. Packaging size: 20T/ 50T / 100T Selected citations: [1] PD-L1 lncRNA splice isoform promotes lung adenocarcinoma progression via enhancing c-Myc activity. Genome Biol. 2021;22(1):104. Published 2021 Apr 13. doi:10.1186/s13059-021-02331-0(IF:13.583) [2] Tumor-derived miR-378a-3p-containing extracellular vesicles promote osteolysis by activating the Dyrk1a/Nfatc1/Angptl2 axis for bone metastasis. Cancer Lett. 2022;526:76-90. doi:10.1016/j.canlet.2021.11.017(IF:8.679) [3] Macroporous organosilicon nanocomposites co-deliver Bcl2-converting peptide and chemotherapeutic agent for synergistic treatment against multidrug resistant cancer. Cancer Lett. 2020;469:340-354. doi:10.1016/j.canlet.2019.10.018(IF:6.508) [4] Nobiletin mitigates hepatocytes death, liver inflammation, and fibrosis in a murine model of NASH through modulating hepatic oxidative stress and mitochondrial dysfunction. J Nutr Biochem. 2022;100:108888. doi:10.1016/j.jnutbio.2021.108888(IF:6.048) [5] eIF4A1 Inhibitor Suppresses Hyperactive mTOR-Associated Tumors by Inducing Necroptosis and G2/M Arrest. Int J Mol Sci. 2022;23(13):6932. Published 2022 Jun 22. doi:10.3390/ijms23136932(IF:5.924)
Features: Applications:
- The reagents in this kit are provided in liquid form for easy use
- This kit is commonly used for flow cytometry
- Under the fluorescence microscope, the color distinction is obvious
- This kit can be used for flow cytometry
- This kit can detect the early apoptosis of cells
- This kit can distinguish between late apoptotic and necrotic cells
.JPG@webp)
Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit - 40305ES
The detection principle of the Annexin V-Alexa Fluor488/PI apoptosis detection kit is as follows: in normal living cells, phosphotidylserine (PS) is located in the inner side of the cell membrane, but in early apoptotic cells, PS flips from the inner side of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin-V is a Ca2+ -dependent phospholipid-binding protein with a molecular weight of 35 to 36KD, which binds with high affinity to PS and binds to the cell membrane of early apoptotic cells through phosphatidylserine exposed on the outside of the cell.
In addition, Propidium Iodide (PI) is provided to distinguish surviving early cells from necrotic or late apoptotic cells. PI is a nucleic acid dye, which can not pass through the intact cell membrane of normal cells or early apoptotic cells but can pass through the cell membrane of late apoptotic and necrotic cells and make the nucleus red. Thus, when Annexin V was combined with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-). In contrast, late apoptotic and necrotic cells were double positive for Alexa Fluor488 and PI (Annexin V+/PI+).
Packaging size: 20T/ 50T / 100T
.JPG@webp)
.JPG@webp)